Proteintech, 80431-2-RR, Phospho-BAD (Ser112) Recombinant monoclonal antibody

Size: 20ul / 100ul The Phospho-BAD (Ser112) (80431-2-RR) by Proteintech is a Recombinant antibody targeting Phospho-BAD (Ser112) in WB, FC (Intra), ELISA applications with reactivity to human samples 80431-2-RR targets Phospho-BAD (Ser112) in WB, FC (Intra), ELISA applications and shows reactivity with human samples. Tested Applications Positive WB detected in: HEK-293 cells Positive FC (Intra) detected in: λ phosphatase treated HEK-293 cells Recommended dilution Western Blot (WB): WB : 1:500-1:2000 Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.13 ug per 10^6 cells in a 100 µl suspension Background Information BAD, a distant member of the BCL-2 family, exerts its death promoting e€ect by hetero-dimerizing with BCL-XL and BCL-2 death antagonists in the mitochondria. The function of the pro-apoptotic molecule BAD is regulated by phosphorylation of two sites, serine-112 (Ser-112) and serine-136 (Ser-136). Phosphorylation at either site results in loss of the ability of BAD to heterodimerize with the survival proteins BCL-XL or BCL-2. Phosphorylated BAD binds to 14-3-3 and is sequestered in the cytoplasm. (PMID: 10597268) Specification Tested Reactivity: human Host / Isotype: Rabbit / IgG Class: Recombinant Type: Antibody Immunogen: Peptide Predict reactive species Full Name: BCL2-associated agonist of cell death Calculated Molecular Weight: 18 kDa Observed Molecular Weight: 25 kDa GenBank Accession Number: BC001901 Gene Symbol: BAD Gene ID (NCBI): 572 Conjugate: Unconjugated Form: Liquid Purification Method: Protein A purification UNIPROT ID: Q92934 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.