Proteintech, 80469-2-RR, MYC tag Recombinant monoclonal antibody

Size: 100ul The MYC tag (80469-2-RR) by Proteintech is a Recombinant antibody targeting MYC tag in WB, IF/ICC, FC (Intra), IP, ELISA applications with reactivity to recombinant protein samples 80469-2-RR targets MYC tag in WB, IF/ICC, FC (Intra), IP, ELISA applications and shows reactivity with recombinant protein samples. Tested Applications Positive WB detected in: Transfected HEK-293T cells Positive IP detected in: Transfected HEK-293 cells Positive IF/ICC detected in: Transfected HEK-293 cells Positive FC (Intra) detected in: Transfected HEK-293 cells Recommended dilution Western Blot (WB): WB : 1:20000-1:100000 Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate Immunofluorescence (IF)/ICC: IF/ICC : 1:125-1:500 Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension Background Information The myc-tag is a short synthetic polypeptide sequence derived from c-MYC protein that can be added to recombinant proteins to enable isolation and study when an antibody is not available. This antibody recognizes the MYC tag EQKLISEEDL. MYC tag is a part of c-MYC protein. So there's weaker 62-65 kDa bands for endogenous c-MYC protein. What is the molecular weight of myc? 1203Da: the ten amino acid myc tag sequence is EQKLISEEDL (in single letter code). What are the applications for myc tag? The addition of the myc tag to a particular protein can be useful when an antibody is not available to the protein of interest. Using recombinant DNA technology, the myc tag can be fused to the protein and then an antibody against the myc tag can be used to probe (PMID: 24490106). This is a reliable method and can be used in a number of different techniques, including purification using chromatography, tracking the protein in localization studies using immunofluorescence, or quantifying levels using Western Blot (PMID: 24490106). What is the structure of myc tag? The c-myc gene from which this tag is derived has a molecular weight of 49kDa, but the myc tag represents only a small portion of the C-terminus of this gene. The short polypeptide sequence can be fused to the N-terminus or the C-terminus of any protein without influencing function, although it is advised to avoid fusing it to a secretory signal. A cleavage site behind this tag is also sometimes engineered to allow removal with a specific protease. Specification Tested Reactivity: recombinant protein Host / Isotype: Rabbit / IgG Class: Recombinant Type: Antibody Immunogen: CatNo: Ag9409 Product name: Recombinant MYC-tag protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 1-50 aa of Sequence: EQKLISEEDLEQKLISEEDLEQKLISEEDL Predict reactive species Full Name: Myc tag Gene Symbol: Myc tag Gene ID (NCBI): 99 Conjugate: Unconjugated Form: Liquid Purification Method: Protein A purfication UNIPROT ID: MYCTAG Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.