Proteintech, 80488-1-RR, Beta Catenin Recombinant monoclonal antibody

Size: 20ul / 100ul The Beta Catenin (80488-1-RR) by Proteintech is a Recombinant antibody targeting Beta Catenin in WB, IHC, IF/ICC, ELISA, ChIP-qPCR applications with reactivity to human, mouse, rat, pig, zebrafish samples 80488-1-RR targets Beta Catenin in WB, IHC, IF/ICC, CoIP, ELISA, ChIP-qPCR applications and shows reactivity with human, mouse, rat, pig, zebrafish samples. Tested Applications Positive WB detected in: A431 cells, zebrafish tissue, HeLa cells, HEK-293 cells, MCF-7 cells, LNCaP cells, HSC-T6 cells, NIH/3T3 cells, 4T1 cells, pig brain tissue Positive IHC detected in: human liver cancer tissue, human breast cancer tissue, human colon cancer tissue, human rectal cancer tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive IF/ICC detected in: MCF-7 cells Positive ChIP-qPCR detected in: HCT 116 cells Recommended dilution Western Blot (WB): WB : 1:5000-1:50000 Immunohistochemistry (IHC): IHC : 1:1000-1:4000 Immunofluorescence (IF)/ICC: IF/ICC : 1:1000-1:4000 CHIP-QPCR: CHIP-QPCR : 1:10-1:100 Background Information β-Catenin, also known as CTNNB1, is an evolutionarily conserved, multifunctional intracellular protein. β-Catenin is a 92-kDa protein, originally identified in cell adherens junctions (AJs) where it functions to bridge the cytoplasmic domain of cadherins to a-catenin and the actin cytoskeleton. Besides its essential role in the AJs, β-catenin is also a key downstream component of the canonical Wnt pathway that plays diverse and critical roles in embryonic development and adult tissue homeostasis. The Wnt/β-catenin pathway is also involved in the activation of other intracellular messengers such as calcium fluxes, JNK, and SRC kinases. Deregulation of β-catenin activity is associated with multiple diseases including cancers. (PMID: 22617422; 18334222) Specification Tested Reactivity: human, mouse, rat, pig, zebrafish Cited Reactivity: human Host / Isotype: Rabbit / IgG Class: Recombinant Type: Antibody Immunogen: Peptide Predict reactive species Full Name: catenin (cadherin-associated protein), beta 1, 88kDa Calculated Molecular Weight: 781 aa, 86 kDa Observed Molecular Weight: 92 kDa GenBank Accession Number: BC058926 Gene Symbol: Beta Catenin Gene ID (NCBI): 1499 ENSEMBL Gene ID: ENSG00000168036 RRID: AB_2918895 Conjugate: Unconjugated Form: Liquid Purification Method: Protein A purification UNIPROT ID: P35222 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.