Proteintech, 80593-1-RR, NRF2/NFE2L2 Recombinant monoclonal antibody

Size: 20ul / 100ul The NRF2/NFE2L2 (80593-1-RR) by Proteintech is a Recombinant antibody targeting NRF2/NFE2L2 in WB, IHC, IF/ICC, FC (Intra), IP, ELISA, ChIP-qPCR applications with reactivity to human samples 80593-1-RR targets NRF2/NFE2L2 in WB, IHC, IF/ICC, FC (Intra), IP, CoIP, ELISA, ChIP-qPCR applications and shows reactivity with human samples. Tested Applications Positive WB detected in: MG132 treated HEK-293 cells, HEK-293 cells, HeLa cells, HepG2 cells Positive IP detected in: HeLa cells Positive IHC detected in: human ovary tumor tissue, Human bowens disease, human pancreas cancer tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive IF/ICC detected in: MG132 treated HepG2 cells, HepG2 cells Positive FC (Intra) detected in: MCF-7 cells Positive ChIP-qPCR detected in: HeLa cells Recommended dilution Western Blot (WB): WB : 1:1000-1:5000 Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate Immunohistochemistry (IHC): IHC : 1:200-1:800 Immunofluorescence (IF)/ICC: IF/ICC : 1:300-1:1200 Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension CHIP-QPCR: CHIP-QPCR : 1:10-1:100 Background Information NRF2, also named as NFE2L2, belongs to the bZIP family and CNC subfamily. It is a transcription activator that binds to antioxidant response (ARE) elements in the promoter regions of target genes. NRF2 is important for the coordinated up-regulation of genes in response to oxidative stress. It may be involved in the transcriptional activation of genes of the beta-globin cluster by mediating enhancer activity of hypersensitive site 2 of the beta-globin locus control region. Nrf2 is a key player in the regulation of genes encoding for many antioxidative response enzymes.The expression of NRF2 may be induced under oxidative stress (PMID:14567983).In lung cancer, Nrf2 activation in malignant cells has been associated with tumor progression and chemotherapy resistance(PMID:20534738). Identifying patients with abnormal NRF2 expression may be important for selection for chemotherapy in NSCLC. As new investigators break into the emerging field of Nrf2 research, confusion regarding the correct migratory pattern of Nrf2 is causing doubts about the accuracy and reproducibility of published results. This letter provides solid evidence that the actually observed molecular weight of Nrf2 is about 70kDa and 95-110 kDa. (PMID: 22703241). Specification Tested Reactivity: human Cited Reactivity: human Host / Isotype: Rabbit / IgG Class: Recombinant Type: Antibody Immunogen: CatNo: Ag9489 Product name: Recombinant human NRF2, NFE2L2 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 256-605 aa of BC011558 Sequence: SILSTEDPNQLTVNSLNSDATVNTDFGDEFYSAFIAEPSISNSMPSPATLSHSLSELLNGPIDVSDLSLCKAFNQNHPESTAEFNDSDSGISLNTSPSVASPEHSVESSSYGDTLLGLSDSEVEELDSAPGSVKQNGPKTPVHSSGDMVQPLSPSQGQSTHVHDAQCENTPEKELPVSPGHRKTPFTKDKHSSRLEAHLTRDELRAKALHIPFPVEKIINLPVVDFNEMMSKEQFNEAQLALIRDIRRRGKNKVAAQNCRKRKLENIVELEQDLDHLKDEKEKLLKEKGENDKSLHLLKKQLSTLYLEVFSMLRDEDGKPYSPSEYSLQQTRDGNVFLVPKSKKPDVKKN Predict reactive species Full Name: nuclear factor (erythroid-derived 2)-like 2 Calculated Molecular Weight: 605 aa, 68 kDa Observed Molecular Weight: 110 kDa, 68 kDa GenBank Accession Number: BC011558 Gene Symbol: NRF2 Gene ID (NCBI): 4780 RRID: AB_2918904 Conjugate: Unconjugated Form: Liquid Purification Method: Protein A purification UNIPROT ID: Q16236 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.