Proteintech, 80694-3-RR, Phospho-YAP1 (Ser397) Recombinant monoclonal antibody

Size: 20ul / 100ul The Phospho-YAP1 (Ser397) (80694-3-RR) by Proteintech is a Recombinant antibody targeting Phospho-YAP1 (Ser397) in WB, FC (Intra), ELISA applications with reactivity to human, mouse samples 80694-3-RR targets Phospho-YAP1 (Ser397) in WB, FC (Intra), ELISA applications and shows reactivity with human, mouse samples. Tested Applications Positive WB detected in: NIH/3T3 cells Positive FC (Intra) detected in: λ phosphatase treated NIH/3T3 cells Recommended dilution Western Blot (WB): WB : 1:1000-1:6000 Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.13 ug per 10^6 cells in a 100 µl suspension Background Information The transcriptional factors Yes-associated protein 1 (YAP1) and PDZ-binding motif (TAZ) act as downstream effectors of the Hippo pathway, and their subcellular location and transcriptional activities are affected by multiple post-translational modifications (PTMs). After phosphorylation, YAP1/TAZ binds to the 14-3-3 protein, which induces cytoplasmic retention of YAP1/TAZ. Moreover, phosphorylated YAP1/TAZ is likely to undergo ubiquitination degradation that is dependent on the β-transducin repeat-containing E3 ubiquitin protein ligase complex. The phosphorylation of YAP at Ser127 promotes its cytoplasmic retention, whereas phosphorylation at Ser397 induces degradation. (PMID: 29682330, PMID: 26039999) Specification Tested Reactivity: human, mouse Host / Isotype: Rabbit / IgG Class: Recombinant Type: Antibody Immunogen: Peptide Predict reactive species Full Name: Yes-associated protein 1, 65kDa Calculated Molecular Weight: 504 aa, 54 kDa Observed Molecular Weight: 75 kDa GenBank Accession Number: BC038235 Gene Symbol: YAP1 Gene ID (NCBI): 10413 Conjugate: Unconjugated Form: Liquid Purification Method: Protein A purification UNIPROT ID: P46937 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.