Proteintech, 80878-6-RR, TrkB Recombinant monoclonal antibody

Size: 20ul / 100ul The TrkB (80878-6-RR) by Proteintech is a Recombinant antibody targeting TrkB in WB, IHC, IF-P, IP, ELISA applications with reactivity to human, mouse, rat samples 80878-6-RR targets TrkB in WB, IHC, IF-P, IP, ELISA applications and shows reactivity with human, mouse, rat samples. Tested Applications Positive WB detected in: rat brain tissue, mouse cerebellum tissue Positive IP detected in: mouse cerebellum tissue Positive IHC detected in: rat brain tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive IF-P detected in: mouse brain tissue Recommended dilution Western Blot (WB): WB : 1:5000-1:50000 Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate Immunohistochemistry (IHC): IHC : 1:500-1:2000 Immunofluorescence (IF)-P: IF-P : 1:200-1:800 Background Information Tropomyosin-related kinase B (TrkB) is a member of the neurotrophic tyrosine receptor kinase (NTRK) family. It is a single-pass type 1 membrane protein consisting of three domains: an extracellular ligand binding domain, a transmembrane domain, and an intracellular tyrosine kinase domain. TrkB is primarily expressed in the central and peripheral nervous systems. TrkB plays an important role in neural development and maintenance by regulating neuronal differentiation, proliferation, survival, migration, neurogenesis, and plasticity. This antibody raised against 41-433aa of human TrkB recognizes the full-length form (140-145 kDa) and truncated form (90-95 kDa) of TrkB (PMID: 23526925; 21039416). Specification Tested Reactivity: human, mouse, rat Cited Reactivity: mouse Host / Isotype: Rabbit / IgG Class: Recombinant Type: Antibody Immunogen: CatNo: Ag3790 Product name: Recombinant human NTRK2 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 41-433 aa of BC031835 Sequence: SRIWCSDPSPGIVAFPRLEPNSVDPENITEIFIANQKRLEIINEDDVEAYVGLRNLTIVDSGLKFVAHKAFLKNSNLQHINFTRNKLTSLSRKHFRHLDLSELILVGNPFTCSCDIMWIKTLQEAKSSPDTQDLYCLNESSKNIPLANLQIPNCGLPSANLAAPNLTVEEGKSITLSCSVAGDPVPNMYWDVGNLVSKHMNETSHTQGSLRITNISSDDSGKQISCVAENLVGEDQDSVNLTVHFAPTITFLESPTSDHHWCIPFTVKGNPKPALQWFYNGAILNESKYICTKIHVTNHTEYHGCLQLDNPTHMNNGDYTLIAKNEYGKDEKQISAHFMGWPGIDDGANPNYPDVIYEDYGTAANDIGDTTNRSNEIPSTDVTDKTGREHLSV Predict reactive species Full Name: neurotrophic tyrosine kinase, receptor, type 2 Calculated Molecular Weight: 822 aa, 92 kDa Observed Molecular Weight: 90-95 kDa GenBank Accession Number: BC031835 Gene Symbol: TrkB Gene ID (NCBI): 4915 RRID: AB_3670492 Conjugate: Unconjugated Form: Liquid Purification Method: Protein A purfication UNIPROT ID: Q16620 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.