Proteintech, 81212-2-RR, Phospho-p38 MAPK (Thr180/Tyr182) Recombinant monoclonal antibody

Size: 20ul / 100ul The Phospho-p38 MAPK (Thr180/Tyr182) (81212-2-RR) by Proteintech is a Recombinant antibody targeting Phospho-p38 MAPK (Thr180/Tyr182) in WB, IHC, ELISA applications with reactivity to human, mouse samples 81212-2-RR targets Phospho-p38 MAPK (Thr180/Tyr182) in WB, IHC, ELISA applications and shows reactivity with human, mouse samples. Tested Applications Positive WB detected in: Anisomycin treated HeLa cells, UV treated NIH/3T3 cells, Anisomycin treated NIH/3T3 cells Positive IHC detected in: human colon cancer tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Recommended dilution Western Blot (WB): WB : 1:2000-1:10000 Immunohistochemistry (IHC): IHC : 1:200-1:800 Background Information A stress-activated serine/threonine protein kinase, p38 mitogen-activated protein kinase (p38 MAPK), belongs to the MAP kinase superfamily. Diverse extracellular stimuli, including ultraviolet light, irradiation, heat shock, high osmotic stress, proinflammatory cytokines and certain mitogens, trigger a stress-regulated protein kinase cascade culminating in activation of p38 MAPK through phosphorylation on a TGY motif within the kinase activation loop. The p38 MAPK undergoes dual phosphorylation at Thr182 and Tyr180 in the Thr-Gly-Tyr activation loop by MAP kinase kinase 6 (MKK6). Upon activation, p38 MAPK phosphorylates multiple substrates, including MAPK activated protein kinase 2 (MAPKAPK2) and activating transcription factor 2 (ATF-2). (PMID: 26901653, PMID: 10807318) Specification Tested Reactivity: human, mouse Cited Reactivity: human Host / Isotype: Rabbit / IgG Class: Recombinant Type: Antibody Immunogen: Peptide Predict reactive species Full Name: mitogen-activated protein kinase 14 Calculated Molecular Weight: 360 aa, 41 kDa Observed Molecular Weight: 38-42 kDa GenBank Accession Number: BC031574 Gene Symbol: p38 MAPK Gene ID (NCBI): 1432 Conjugate: Unconjugated Form: Liquid Purification Method: Protein A purfication UNIPROT ID: Q16539 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.