Proteintech, 81281-1-RR, HO-1 Recombinant monoclonal antibody

Size: 20ul / 100ul The HO-1 (81281-1-RR) by Proteintech is a Recombinant antibody targeting HO-1 in WB, IP, ELISA applications with reactivity to human, rat samples 81281-1-RR targets HO-1 in WB, IHC, IP, ELISA applications and shows reactivity with human, rat samples. Tested Applications Positive WB detected in: A549 cells, HeLa cells, HepG2 cells, U2OS cells, HSC-T6 cells, rat spleen tissue Positive IP detected in: HeLa cells Recommended dilution Western Blot (WB): WB : 1:5000-1:50000 Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate Background Information Heme oxygenase (HMOX1) catalyzes the first and rate-limiting step in the degradation of heme to yield equimolar quantities of biliverdin Ixa, carbon monoxide (CO), and iron. It has 3 isoforms: HO-1 is highly inducible, whereas HO-2 and HO-3 are constitutively expressed (PMID:10194478). Heme oxygenase-1 (HO-1) is expressed in many tissues and vascular smooth muscle cells, and endothelial cells (PMID:15451051) and has been identified as an important endogenous protective factor induced in many cell types by various stimulants, such as hemolysis, infiammatory cytokines,oxidative stress, heat shock, heavy metals, and endotoxin (PMID: 11522663). Specification Tested Reactivity: human, rat Cited Reactivity: human, rat, pig Host / Isotype: Rabbit / IgG Class: Recombinant Type: Antibody Immunogen: CatNo: Ag1190 Product name: Recombinant human HO-1 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 1-288 aa of BC001491 Sequence: MERPQPDSMPQDLSEALKEATKEVHTQAENAEFMRNFQKGQVTRDGFKLVMASLYHIYVALEEEIERNKESPVFAPVYFPEELHRKAALEQDLAFWYGPRWQEVIPYTPAMQHYVKRLHEVGRTEPELLVAHAYTRYLGDLSGGQVLKKIAQKALDLPSSGEGLAFFTFPNIASATKFKQLYRSRMNSLEMTPAVRQRVIEEAKTAFLLNIQLFEELQELLTHDTKDQSPSRAPGLRQRASNKVQDSAPVETPRGKPPLNTRSQAPLLRWVLTLSFLVATVAVGLYAM Predict reactive species Full Name: heme oxygenase (decycling) 1 Calculated Molecular Weight: 33 kDa Observed Molecular Weight: 33 kDa GenBank Accession Number: BC001491 Gene Symbol: HO-1 Gene ID (NCBI): 3162 RRID: AB_2923713 Conjugate: Unconjugated Form: Liquid Purification Method: Protein A purification UNIPROT ID: P09601 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.