Proteintech, 81304-1-RR, Phospho-MEK1 (Thr386) Recombinant monoclonal antibody

Size: 20ul / 100ul The Phospho-MEK1 (Thr386) (81304-1-RR) by Proteintech is a Recombinant antibody targeting Phospho-MEK1 (Thr386) in WB, IF/ICC, FC (Intra), ELISA applications with reactivity to human samples 81304-1-RR targets Phospho-MEK1 (Thr386) in WB, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human samples. Tested Applications Positive WB detected in: HeLa cells, λ phosphatase treated HeLa cells Positive IF/ICC detected in: λ phosphatase treated HeLa cells Positive FC (Intra) detected in: λ phosphatase treated HeLa cells Recommended dilution Western Blot (WB): WB : 1:5000-1:50000 Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500 Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.06 ug per 10^6 cells in a 100 µl suspension Background Information MAP2K1 encodes MAPK1, also known as MEK1. MEK1 variants can enhance MEK1 expression and ERK1 phosphorylation that together lead to continuous activation of MEK/ERK signaling pathway. MEK1 bind directly to ERK2 through a region in the N terminus of MEK. In addition, a proline-rich (PR) regulatory sequence in MEK is also involved in MEK-ERK association and signal propagation. The coupling between MEK1 and ERK2 is enhanced through phosphorylation on S298 in the MEK1 PR region, whereas phosphorylation on MEK1 T292 releases the complex. MEK1 T292 is a substrate of ERK2, but the site is also phosphorylated at a basal level when ERK2 is inhibited, suggesting several regulators of this site . Although the S298 site in MEK2 has been conserved, it lacks the T292 phosphorylation site, and it is not a substrate of PAK1. (PMID: 31972311, PMID: 17928366, PMID: 22177953) Specification Tested Reactivity: human Cited Reactivity: human, mouse Host / Isotype: Rabbit / IgG Class: Recombinant Type: Antibody Immunogen: Peptide Predict reactive species Full Name: mitogen-activated protein kinase kinase 1 Calculated Molecular Weight: 43 kDa Observed Molecular Weight: 40-50 kDa GenBank Accession Number: BC139729 Gene Symbol: MEK1 Gene ID (NCBI): 5604 ENSEMBL Gene ID: ENSG00000169032 RRID: AB_2923714 Conjugate: Unconjugated Form: Liquid Purification Method: Protein A purification UNIPROT ID: Q02750 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.