Proteintech, 81433-1-RR, Phospho-SMAD1 (Ser214) Recombinant monoclonal antibody

Size: 20ul / 100ul The Phospho-SMAD1 (Ser214) (81433-1-RR) by Proteintech is a Recombinant antibody targeting Phospho-SMAD1 (Ser214) in WB, ELISA applications with reactivity to human, mouse samples 81433-1-RR targets Phospho-SMAD1 (Ser214) in WB, ELISA applications and shows reactivity with human, mouse samples. Tested Applications Positive WB detected in: UV treated A431 cells, UV treated NIH/3T3 cells, Calyculin A treated HepG2 cells Recommended dilution Western Blot (WB): WB : 1:2000-1:16800 Background Information SMAD family member 1 (Smad1) have been involved in metastatic progression of many cancer types. Smad1 can be induced by many tumor-stimulating cytokines such as the bone morphogenetic protein 2 (BMP2) and TNFα and plays important roles in cell invasion and metastasis. BMP2 signalling is initiated by binding to its specific receptors, which leads to in the phosphorylation and nuclear translocation of Smad1. Translocated Smad1 then modulates the expression of downstream osteogenic genes. (PMID: 32954678, PMID: 30008908) Specification Tested Reactivity: human, mouse Host / Isotype: Rabbit / IgG Class: Recombinant Type: Antibody Immunogen: Peptide Predict reactive species Full Name: SMAD family member 1 Calculated Molecular Weight: 52 kDa Observed Molecular Weight: 52 kDa GenBank Accession Number: BC001878 Gene Symbol: SMAD1 Gene ID (NCBI): 4086 RRID: AB_2923717 Conjugate: Unconjugated Form: Liquid Purification Method: Protein A purification UNIPROT ID: Q15797 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.