Proteintech, 81504-1-RR, ENO1/2/3 Recombinant monoclonal antibody

Size: 20ul / 100ul The ENO1/2/3 (81504-1-RR) by Proteintech is a Recombinant antibody targeting ENO1/2/3 in WB, IHC, IF/ICC, ELISA applications with reactivity to human, mouse, rat, pig samples 81504-1-RR targets ENO1/2/3 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat, pig samples. Tested Applications Positive WB detected in: HEK-293 cells, HeLa cells, HepG2 cells, PC-3 cells, MCF-7 cells, Jurkat cells, mouse brain tissue, rat brain tissue, pig brain tissue Positive IHC detected in: mouse brain tissue, human colon cancer tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive IF/ICC detected in: HepG2 cells Recommended dilution Western Blot (WB): WB : 1:5000-1:50000 Immunohistochemistry (IHC): IHC : 1:500-1:2000 Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500 Background Information Enolase (ENO), also known as 2-phospho-D-glycerate hydrolase, is a metalloenzyme that catalyzes the dehydration of 2-phospho-D-glycerate into phosphoenolpyruvate in the glycolytic pathway. There are three different ENO isoforms in higher eukaryotes, including ENO1 or α-ENO, ENO2 or γ-ENO, and ENO3 or β-ENO. Furthermore, the expression of the different ENO isoforms is tissue specificity. ENO1 is expressed in almost all kinds of tissue, whereas ENO3 is restricted to muscle tissues and ENO2 is described as a neuron- and neuroendocrine tissue-specific enolase. ENO1 serves multifunctional roles in physiological and pathological processes. (PMID: 32256815). This antibody can recognize ENO1, ENO2, and ENO3. Specification Tested Reactivity: human, mouse, rat, pig Host / Isotype: Rabbit / IgG Class: Recombinant Type: Antibody Immunogen: CatNo: Ag1692 Product name: Recombinant human ENO1 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 2-434 aa of BC015641 Sequence: SILKIHAREIFDSRGNPTVEVDLFTSKGLFRAAVPSGASTGIYEALELRDNDKTRYMGKGVSKAVEHINKTIAPALVSKKLNVTEQEKIDKLMIEMDGTENKSKFGANAILGVSLAVCKAGAVEKGVPLYRHIADLAGNSEVILPVPAFNVINGGSHAGNKLAMQEFMILPVGAANFREAMRIGAEVYHNLKNVIKEKYGKDATNVGDEGGFAPNILENKEGLELLKTAIGKAGYTDKVVIGMDVAASEFFRSGKYDLDFKSPDDPSRYISPDQLADLYKSFIKDYPVVSIEDPFDQDDWGAWQKFTASAGIQVVGDDLTVTNPKRIAKAVNEKSCNCLLLKVNQIGSVTESLQACKLAQANGWGVMVSHRSGETEDTFIADLVVGLCTGQIKTGAPCRSERLAKYNQLLRIEEELGSKAKFAGRNFRNPLAK Predict reactive species Full Name: enolase 1, (alpha) Calculated Molecular Weight: 47 kDa Observed Molecular Weight: 47 kDa GenBank Accession Number: BC015641 Gene Symbol: ENO1 Gene ID (NCBI): 2023 ENSEMBL Gene ID: ENSG00000074800 RRID: AB_2935557 Conjugate: Unconjugated Form: Liquid Purification Method: Protein A purification UNIPROT ID: P06733 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.