Proteintech, 81584-5-RR, SNAI1 Recombinant monoclonal antibody

Size: 20ul / 100ul The SNAI1 (81584-5-RR) by Proteintech is a Recombinant antibody targeting SNAI1 in WB, FC (Intra), ELISA applications with reactivity to human samples 81584-5-RR targets SNAI1 in WB, FC (Intra), ELISA applications and shows reactivity with human samples. Tested Applications Positive WB detected in: A549 cells, MCF-7 cells, BxPC-3 cells Positive FC (Intra) detected in: A549 cells Recommended dilution Western Blot (WB): WB : 1:5000-1:50000 Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension Background Information SNAI1, a member of SNAI1 family of protein, participates in the epithelial to mesenchymal transition(EMT) and formation and maintenance of embryonic mesoderm. The snail family share a common structural, that a highly conserved C-terminal region containing a zinc finger transcription factor. SNAI1 interacts with other corepressor, such as Ajuba, PRMT5 and SIN3a or HDAC1 and 2, to repress the target gene. As the phosphorylation modification of SNAI1 protein, the range of molecular weight of SNAI1 is about 25-30 kDa (PMID: 22276203 ). Once phosphorylated (probably on Ser-107, Ser-111, Ser-115 and Ser-119) it is exported from the nucleus to the cytoplasm where subsequent phosphorylation of the destruction motif and ubiquitination involving BTRC occurs. Specification Tested Reactivity: human Host / Isotype: Rabbit / IgG Class: Recombinant Type: Antibody Immunogen: CatNo: Ag24248 Product name: Recombinant human SNAI1 protein Source: e coli. -derived, PET28a Tag: 6*His Domain: 22-164 aa of BC012910 Sequence: LQDSNPEFTFQQPYDQAHLLAAIPPPEILNPTASLPMLIWDSVLAPQAQPIAWASLRLQESPRVAELTSLSDEDSGKGSQPPSPPSPAPSSFSSTSVSSLEAEAYAAFPGLGQVPKQLAQLSEAKDLQARKAFNCKYCNKEYL Predict reactive species Full Name: snail homolog 1 (Drosophila) Calculated Molecular Weight: 264 aa, 29 kDa Observed Molecular Weight: 30 kDa GenBank Accession Number: BC012910 Gene Symbol: SNAI1 Gene ID (NCBI): 6615 RRID: AB_3670500 Conjugate: Unconjugated Form: Liquid Purification Method: Protein A purfication UNIPROT ID: O95863 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.