Proteintech, 81667-2-RR, Phospho-JUN (Thr91/93) Recombinant monoclonal antibody

Size: 20ul / 100ul The Phospho-JUN (Thr91/93) (81667-2-RR) by Proteintech is a Recombinant antibody targeting Phospho-JUN (Thr91/93) in WB, FC (Intra), ELISA applications with reactivity to human, mouse samples 81667-2-RR targets Phospho-JUN (Thr91/93) in WB, FC (Intra), ELISA applications and shows reactivity with human, mouse samples. Tested Applications Positive WB detected in: UV treated NIH/3T3 cells Positive FC (Intra) detected in: UV treated NIH/3T3 cells Recommended dilution Western Blot (WB): WB : 1:2000-1:16000 Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.13 ug per 10^6 cells in a 100 µl suspension Background Information JUN is also named as c-Jun and AP1, belongs to the bZIP family and Jun subfamily. JUN, the most extensively studied protein of the activator protein-1 (AP-1) complex, is involved in numerous cell activities, such as proliferation, apoptosis, survival, tumorigenesis and tissue morphogenesis.The phosphorylation of c-Jun by Plk3 immunoprecipitates was not altered by the pre-removal of JNK from the cell lysates. In addition, the effect of UV irradiation-induced phosphorylation of c-Jun and apoptosis were not significantly affected by knockdown of JNK mRNA. (PMID: 22180088, PMID: 17804415) Specification Tested Reactivity: human, mouse Host / Isotype: Rabbit / IgG Class: Recombinant Type: Antibody Immunogen: Peptide Predict reactive species Full Name: jun oncogene Calculated Molecular Weight: 331 aa, 36 kDa Observed Molecular Weight: 48 kDa GenBank Accession Number: BC068522 Gene Symbol: JUN Gene ID (NCBI): 3725 RRID: AB_3670501 Conjugate: Unconjugated Form: Liquid Purification Method: Protein A purfication UNIPROT ID: P05412 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.