Proteintech, 81785-1-RR, Phospho-TAK1 (Thr187) Recombinant monoclonal antibody

Size: 20ul / 100ul The Phospho-TAK1 (Thr187) (81785-1-RR) by Proteintech is a Recombinant antibody targeting Phospho-TAK1 (Thr187) in WB, FC (Intra), ELISA applications with reactivity to human samples 81785-1-RR targets Phospho-TAK1 (Thr187) in WB, FC (Intra), ELISA applications and shows reactivity with human samples. Tested Applications Positive WB detected in: Calyculin A treated HEK-293 cells, Calyculin A treated HeLa cells Positive FC (Intra) detected in: Calyculin A treated HEK-293 cells Recommended dilution Western Blot (WB): WB : 1:20000-1:100000 Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.13 ug per 10^6 cells in a 100 µl suspension Background Information MAP3K7(Mitogen-activated protein kinase kinase kinase 7) is also named TAK1 and belongs to the MAP kinase kinase kinase subfamily. It plays an important role in the cascades of cellular responses evoked by changes in the environment. It has been linked to interleukin-1 receptor and tumor necrosis factor receptor signaling (PMID: 16186825). It has 4 isoforms (53-55 kDa; 64-70 kDa and 75-80 kDa)produced by alternative splicing. Specification Tested Reactivity: human Host / Isotype: Rabbit / IgG Class: Recombinant Type: Antibody Immunogen: Peptide Predict reactive species Full Name: mitogen-activated protein kinase kinase kinase 7 Calculated Molecular Weight: 579 aa, 64 kDa Observed Molecular Weight: 75-85 kDa GenBank Accession Number: BC017715 Gene Symbol: TAK1 Gene ID (NCBI): 6885 RRID: AB_3085357 Conjugate: Unconjugated Form: Liquid Purification Method: Protein A purification UNIPROT ID: O43318 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.