Proteintech, 81892-1-RR, USP9X Recombinant monoclonal antibody

Size: 20ul / 100ul The USP9X (81892-1-RR) by Proteintech is a Recombinant antibody targeting USP9X in WB, IF/ICC, IP, ELISA applications with reactivity to human, mouse, rat samples 81892-1-RR targets USP9X in WB, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples. Tested Applications Positive WB detected in: HEK-293 cells, HeLa cells, A549 cells, K-562 cells, Jurkat cells, mouse brain tissue, rat brain tissue Positive IP detected in: HEK-293 cells Positive IF/ICC detected in: HeLa cells Recommended dilution Western Blot (WB): WB : 1:5000-1:50000 Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500 Background Information USP9X, also named as DFFRX, FAM and USP9, belongs to the peptidase C19 family. It may function as a ubiquitin-protein or polyubiquitin hydrolase involved both in the processing of ubiquitin precursors and of ubiquitinated proteins. It plays an important role regulatory role at the level of protein turnover by preventing degradation of proteins through the removal of conjugated ubiquitin. USP9X is an essential component of TGF-beta/BMP signaling cascade. Deubiqitination of SMAD4 by USP9X restores its competence to mediate TGF-beta signaling. USP9X regulates chromosome alignment and segregation in mitosis by regulating the localization of BIRC5/survivin to mitotic centromeres. The antibody is specific to USP9X. It has no cross reaction to USP9Y. Specification Tested Reactivity: human, mouse, rat Cited Reactivity: human Host / Isotype: Rabbit / IgG Class: Recombinant Type: Antibody Immunogen: Peptide Predict reactive species Full Name: ubiquitin specific peptidase 9, X-linked Calculated Molecular Weight: 292 kDa Observed Molecular Weight: 260-290 kDa GenBank Accession Number: NM_001039590 Gene Symbol: USP9X Gene ID (NCBI): 8239 RRID: AB_2935588 Conjugate: Unconjugated Form: Liquid Purification Method: Protein A purification UNIPROT ID: Q93008 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.