Proteintech, 82090-2-RR, Phospho-MLKL (Ser358) Recombinant monoclonal antibody

Size: 20ul / 100ul The Phospho-MLKL (Ser358) (82090-2-RR) by Proteintech is a Recombinant antibody targeting Phospho-MLKL (Ser358) in WB, ELISA applications with reactivity to human samples 82090-2-RR targets Phospho-MLKL (Ser358) in WB, ELISA applications and shows reactivity with human samples. Tested Applications Positive WB detected in: TNF alpha and Calyculin A treated HT-29 cells Recommended dilution Western Blot (WB): WB : 1:5000-1:50000 Background Information Mixed lineage kinase domain like pseudokinase (MLKL), belongs to the protein kinase superfamily. One common method for inducing necroptosis is by treating cells grown in culture with a mixture composed of TNF-α, a Smac-mimetic, that degrades cellular inhibitors of apoptosis (cIAPs) and the pancaspase inhibitor Z-VAD-FMK. This mixture is abbreviated as T/S/Z. It drives necrosome complex formation and RIPK3-dependent phosphorylation of Thr-357 and Ser-358 located within the C-terminal kinase-like domain of human MLKL. (PMID: 28878015) Specification Tested Reactivity: human Cited Reactivity: mouse Host / Isotype: Rabbit / IgG Class: Recombinant Type: Antibody Immunogen: Peptide Predict reactive species Full Name: mixed lineage kinase domain-like Calculated Molecular Weight: 54 kDa Observed Molecular Weight: 54 kDa GenBank Accession Number: XM_005255834 Gene Symbol: MLKL Gene ID (NCBI): 197259 RRID: AB_3670515 Conjugate: Unconjugated Form: Liquid Purification Method: Protein A purfication UNIPROT ID: Q8NB16 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.