Proteintech, 82309-1-RR, Phospho-RASGRF1 (Ser927) Recombinant monoclonal antibody

Size: 20ul / 100ul The Phospho-RASGRF1 (Ser927) (82309-1-RR) by Proteintech is a Recombinant antibody targeting Phospho-RASGRF1 (Ser927) in WB, ELISA applications with reactivity to human samples 82309-1-RR targets Phospho-RASGRF1 (Ser927) in WB, ELISA applications and shows reactivity with human samples. Tested Applications Positive WB detected in: Forskolin treated HeLa cells Recommended dilution Western Blot (WB): WB : 1:1000-1:5000 Background Information The RASGRF1 exchange factor, which is also known as CDC25 in Saccharomyces cerevisiae, promotes the exchange of Ras-bound GDP by GTP, is known to play central roles in pathways of cellular growth and differentiation. The serine residue at position 916 in mouse Ras-GRF1 is a PKA phosphorylation site, and the corresponding human residue is serine 927. It's reported that phosphorylation of serine 916 is necessary but not sufficient for maximal activation of mouse RASGRF1. The calculated MW of non-phosphorylated RASGRF1 is 145 kDa. 82309-1-RR can detect a band around 145 kDa, and can detect the increasing phosphorylation level after Forskolin treated.(PMID:15853814, 10601308) Specification Tested Reactivity: human Cited Reactivity: human Host / Isotype: Rabbit / IgG Class: Recombinant Type: Antibody Immunogen: Peptide Predict reactive species Full Name: Ras protein-specific guanine nucleotide-releasing factor 1 Calculated Molecular Weight: 134 kDa Observed Molecular Weight: 145 kDa GenBank Accession Number: BC040275 Gene Symbol: RASGRF1 Gene ID (NCBI): 5923 RRID: AB_3085358 Conjugate: Unconjugated Form: Liquid Purification Method: Protein A purification UNIPROT ID: Q13972 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.