Proteintech, 82636-2-RR, RUNX2 Recombinant monoclonal antibody

Size: 20ul / 100ul The RUNX2 (82636-2-RR) by Proteintech is a Recombinant antibody targeting RUNX2 in WB, IP, ELISA, ChIP-qPCR applications with reactivity to human, mouse samples 82636-2-RR targets RUNX2 in WB, IHC, IF, IP, CoIP, ELISA, ChIP-qPCR applications and shows reactivity with human, mouse samples. Tested Applications Positive WB detected in: PC-3 cells, Saos-2 cells, C2C12 cells Positive IP detected in: ROS1728 cells Positive ChIP-qPCR detected in: Saos-2 cells Recommended dilution Western Blot (WB): WB : 1:2000-1:10000 Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate CHIP-QPCR: CHIP-QPCR : 1:10-1:100 Background Information RUNX2, also named as AML3, CBFA1, OSF2 and PEBP2A, is a Transcription factor which involved in osteoblastic differentiation and skeletal morphogenesis. It is essential for the maturation of osteoblasts and both intramembranous and endochondral ossification. CBF binds to the core site, 5'-PYGPYGGT-3', of a number of enhancers and promoters, including murine leukemia virus, polyomavirus enhancer, T-cell receptor enhancers, osteocalcin, osteopontin, bone sialoprotein, alpha 1(I) collagen, LCK, IL-3 and GM-CSF promoters By similarity. RUNX2 inhibits MYST4-dependent transcriptional activation. Specification Tested Reactivity: human, mouse Cited Reactivity: human, mouse, rat Host / Isotype: Rabbit / IgG Class: Recombinant Type: Antibody Immunogen: Peptide Predict reactive species Full Name: runt-related transcription factor 2 Calculated Molecular Weight: 57 kDa Observed Molecular Weight: 57-60 kDa GenBank Accession Number: NM_004348 Gene Symbol: RUNX2 Gene ID (NCBI): 860 RRID: AB_3086495 Conjugate: Unconjugated Form: Liquid Purification Method: Protein A purification UNIPROT ID: Q13950 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.