Proteintech, 82674-10-RR, Phospho-STAT1 (Tyr701) Recombinant monoclonal antibody

Size: 20ul / 100ul The Phospho-STAT1 (Tyr701) (82674-10-RR) by Proteintech is a Recombinant antibody targeting Phospho-STAT1 (Tyr701) in WB, FC (Intra), ELISA, ChIP-qPCR applications with reactivity to human samples 82674-10-RR targets Phospho-STAT1 (Tyr701) in WB, FC (Intra), ELISA, ChIP-qPCR applications and shows reactivity with human samples. Tested Applications Positive WB detected in: IFN gamma treated THP-1 cells Positive FC (Intra) detected in: IFN gamma treated THP-1 cells Positive ChIP-qPCR detected in: IFN-γ (50 ng/ml, 30min) HT-1080 cells Recommended dilution Western Blot (WB): WB : 1:2000-1:10000 Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.13 ug per 10^6 cells in a 100 µl suspension CHIP-QPCR: CHIP-QPCR : 1:10-1:100 Background Information STAT1 (signal transducers and activators of transcription 1) is a member of the STAT protein family. STAT family members are phosphorylated by the receptor associated kinases, and then form homo-or heterodimers that translocate to the cell nucleus where they act as transcription activators. The phosphorylation of STAT1 at Tyr701 promotes the dimerization of STAT1 and its translocation into the nucleus where it binds the GAS promoter to allow the transcription of inflammatory response genes(PMID: 31408005). The transcript of STAT1 undergoes alternative splicing, resulting in two isoforms: STAT1α (91 kDa) and STAT1β (84 kDa)(PMID: 20159032). Specification Tested Reactivity: human Host / Isotype: Rabbit / IgG Class: Recombinant Type: Antibody Immunogen: Peptide Predict reactive species Full Name: signal transducer and activator of transcription 1, 91kDa Calculated Molecular Weight: 83 kDa Observed Molecular Weight: 84-91 kDa GenBank Accession Number: BC002704 Gene Symbol: STAT1 Gene ID (NCBI): 6772 RRID: AB_3085362 Conjugate: Unconjugated Form: Liquid Purification Method: Protein A purification UNIPROT ID: P42224 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.