Proteintech, 82705-8-RR, GOLGA2/GM130 Recombinant monoclonal antibody

Size: 20ul / 100ul The GOLGA2/GM130 (82705-8-RR) by Proteintech is a Recombinant antibody targeting GOLGA2/GM130 in WB, IHC, IF/ICC, ELISA applications with reactivity to human, rat samples 82705-8-RR targets GOLGA2/GM130 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, rat samples. Tested Applications Positive WB detected in: HeLa cells, HepG2 cells, HEK-293 cells, Jurkat cells, K-562 cells, HSC-T6 cells Positive IHC detected in: rat kidney tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive IF/ICC detected in: HeLa cells Recommended dilution Western Blot (WB): WB : 1:1000-1:8400 Immunohistochemistry (IHC): IHC : 1:250-1:1000 Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800 Background Information GOLGA2, also known as GM130, is a 130 kDa cis-Golgi matrix protein which is one component of the detergent and salt resistant Golgi matrix. It is a peripheral membrane protein highly bound to Golgi membrane and localized mainly at the cytoplasmic face of cis-Golgi membrane. Together with its interacting partner proteins, including p115, giantin, GRASP65, and Rab GTPase, GOLGA2/GM130 is involved in the regulation of ER-to-Golgi transport and also in the maintenance of the Golgi structure. Emerging evidence suggest that the GOLGA2/GM130 has potential roles in the control of glycosylation, cell cycle progression, and higher order cell functions such as cell polarization and directed cell migration. (PMID: 20197635) Specification Tested Reactivity: human, rat Host / Isotype: Rabbit / IgG Class: Recombinant Type: Antibody Immunogen: CatNo: Ag1848 Product name: Recombinant human GM130;GOLGA2 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 361-678 aa of BC014188 Sequence: LRAQLSLMAHPGEGDGLDREEEEDEEEEEEEAVAVPQPMPSIPEDLESREAMVAFFNSAVASAEEEQARLRGQLKEQRVRCRRLAHLLASAQKEPEAAAPAPGTGGDSVCGETHRALQGAMEKLQSRFMELMQEKADLKERVEELEHRCIQLSGETDTIGEYIALYQSQRAVLKERHREKEEYISRLAQDKEEMKVKLLELQELVLRLVGDRNEWHGRFLAAAQNPADEPTSGAPAPQELGAANQQGDLCEVSLAGSVEPAQGEAREGSPRDNPTAQQIMQLLREMQNPRERPGLGSNPCIPFFYRADENDEVKITVI Predict reactive species Full Name: golgi autoantigen, golgin subfamily a, 2 Calculated Molecular Weight: 111 kDa Observed Molecular Weight: 130-140 kDa GenBank Accession Number: BC014188 Gene Symbol: GM130 Gene ID (NCBI): 2801 RRID: AB_3086513 Conjugate: Unconjugated Form: Liquid Purification Method: Protein A purification UNIPROT ID: Q08379 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.