Proteintech, 82714-5-RR, ACTA1-Specific Recombinant monoclonal antibody

Size: 20ul / 100ul The ACTA1-Specific (82714-5-RR) by Proteintech is a Recombinant antibody targeting ACTA1-Specific in WB, IHC, IF-P, ELISA applications with reactivity to human, mouse, rat, pig, chicken samples 82714-5-RR targets ACTA1-Specific in WB, IHC, IF-P, ELISA applications and shows reactivity with human, mouse, rat, pig, chicken samples. Tested Applications Positive WB detected in: mouse skeletal muscle tissue, rat skeletal muscle tissue, pig skeletal muscle tissue, chicken skeletal muscle tissue Positive IHC detected in: mouse skeletal muscle tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive IF-P detected in: mouse skeletal muscle tissue Recommended dilution Western Blot (WB): WB : 1:5000-1:50000 Immunohistochemistry (IHC): IHC : 1:500-1:2000 Immunofluorescence (IF)-P: IF-P : 1:50-1:500 Background Information Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells. The ACTA1 gene encodes skeletal muscle alpha-actin, the principal actin isoform in adult skeletal muscle, which interacts with a variety of proteins to produce the force for muscle contraction. There is only the N-termninal 8 amino acids difference among the actin family members. This antibody is specific to the ACTA1. It does not cross-react with other actin isoforms. Specification Tested Reactivity: human, mouse, rat, pig, chicken Host / Isotype: Rabbit / IgG Class: Recombinant Type: Antibody Immunogen: Peptide Predict reactive species Full Name: actin, alpha 1, skeletal muscle Calculated Molecular Weight: 42 kDa Observed Molecular Weight: 39-42 kDa GenBank Accession Number: NM_001100 Gene Symbol: ACTA1 Gene ID (NCBI): 58 RRID: AB_3086515 Conjugate: Unconjugated Form: Liquid Purification Method: Protein A purification UNIPROT ID: P68133 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.