Proteintech, 82738-4-RR, TOP2A Recombinant monoclonal antibody

Size: 20ul / 100ul The TOP2A (82738-4-RR) by Proteintech is a Recombinant antibody targeting TOP2A in WB, IHC, IF/ICC, IP, ELISA applications with reactivity to human samples 82738-4-RR targets TOP2A in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human samples. Tested Applications Positive WB detected in: K-562 cells, HepG2 cells, HeLa cells, HEK-293 cells, A431 cells, Jurkat cells Positive IP detected in: HeLa cells Positive IHC detected in: human tonsillitis tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive IF/ICC detected in: A431 cells Recommended dilution Western Blot (WB): WB : 1:2000-1:10000 Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate Immunohistochemistry (IHC): IHC : 1:2000-1:8000 Immunofluorescence (IF)/ICC: IF/ICC : 1:250-1:1000 Background Information TOP2A, also named as TOP2, belongs to the type II topoisomerase family. Topoisomerase II makes double-strand breaks. TOP2 control the topological states of DNA by transient breakage and subsequent rejoining of DNA strands. TOP2A may play a role in regulating the period length of ARNTL/BMAL1 transcriptional oscillation. DNA topoisomerase IIa (170 kDa, TOP2a/170) is essential in proliferating cells by resolving DNA topological entanglements during chromosome condensation, replication, and segregation. Specification Tested Reactivity: human Host / Isotype: Rabbit / IgG Class: Recombinant Type: Antibody Immunogen: CatNo: Ag19551 Product name: Recombinant human TOP2A protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 1187-1531 aa of BC140791 Sequence: QDEQVGLPGKGGKAKGKKTQMAEVLPSPRGQRVIPRITIEMKAEAEKKNKKKIKNENTEGSPQEDGVELEGLKQRLEKKQKREPGTKTKKQTTLAFKPIKKGKKRNPWSDSESDRSSDESNFDVPPRETEPRRAATKTKFTMDLDSDEDFSDFDEKTDDEDFVPSDASPPKTKTSPKLSNKELKPQKSVVSDLEADDVKGSVPLSSSPPATHFPDETEITNPVPKKNVTVKKTAAKSQSSTSTTGAKKRAAPKGTKRDPALNSGVSQKPDPAKTKNRRKRKPSTSDDSDSNFEKIVSKAVTSKKSKGESDDFHMDFDSAVAPRAKSVRAKKPIKYLEESDEDDLF Predict reactive species Full Name: topoisomerase (DNA) II alpha 170kDa Calculated Molecular Weight: 1531 aa, 174 kDa Observed Molecular Weight: ~170 kDa GenBank Accession Number: BC140791 Gene Symbol: TOP2A Gene ID (NCBI): 7153 RRID: AB_3086521 Conjugate: Unconjugated Form: Liquid Purification Method: Protein A purification UNIPROT ID: P11388 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.