Proteintech, 82744-2-RR, ADAMTS4 Recombinant monoclonal antibody

Size: 20ul / 100ul The ADAMTS4 (82744-2-RR) by Proteintech is a Recombinant antibody targeting ADAMTS4 in WB, FC (Intra), ELISA applications with reactivity to human, mouse, rat samples 82744-2-RR targets ADAMTS4 in WB, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat samples. Tested Applications Positive WB detected in: THP-1 cells, HEK-293T cells, SH-SY5Y cells, Caco-2 cells, mouse brain tissue, rat brain tissue Positive FC (Intra) detected in: U2OS cells Recommended dilution Western Blot (WB): WB : 1:1000-1:5000 Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension Background Information The ADAMTS (a disintegrin-like and metalloproteinase with thrombospondin motifs) is a family of extracellular metalloproteinases mediating diverse functions including matrix degradation, blood coagulation and angiogenesis. The ADAMTS family constitutes a group of proteins composed of 19 enzymes and 7 ADAMTS-like proteins. ADAMTS4 is a well-known proteoglycanase and has angiomodulatory properties. The 837 amino acid long human ADAMTS4 protein contains a long signal peptide, a prodomain, a metalloproteinase catalytic domain with zinc-binding motif, a disintegrin-like domain, a central TSR motif, followed by a cysteine-rich region with ten conserved cysteine residues, and a spacer domain. The full-length proform human ADAMTS4 (zymogen form) has been described to be 90 kDa. In extracellular matrix (ECM), ADAMTS4 undergoes further C-terminal cleavage at Lys694-Phe695 and Thr581-Phe582, respectively, to generate two other truncated forms-53 and 40 kDa (PMID:12202483). Specification Tested Reactivity: human, mouse, rat Host / Isotype: Rabbit / IgG Class: Recombinant Type: Antibody Immunogen: CatNo: Ag2443 Product name: Recombinant human ADAMTS4 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 2-339 aa of BC030812 Sequence: SQTGSHPGRGLAGRWLWGAQPCLLLPIVPLSWLVWLLLLLLASLLPSARLASPLPREEEIVFPEKLNGSVLPGSGAPARLLCRLQAFGETLLLELEQDSGVQVEGLTVQYLGQAPELLGGAEPGTYLTGTINGDPESVASLHWDGGALLGVLQYRGAELHLQPLEGGTPNSAGGPGAHILRRKSPASGQGPMCNVKAPLGSPSPRPRRAKRFASLSRFVETLVVADDKMAAFHGAGLKRYLLTVMAAAAKAFKHPSIRNPVSLVVTRLVILGSGEEGPQVGPSAAQTLRSFCAWQRGLNTPEDSDPDHFDTAILFTRQVRPQSAPQAMHCTILRSATT Predict reactive species Full Name: ADAM metallopeptidase with thrombospondin type 1 motif, 4 Calculated Molecular Weight: 837 aa, 90 kDa Observed Molecular Weight: 90 kDa GenBank Accession Number: BC030812 Gene Symbol: ADAMTS4 Gene ID (NCBI): 9507 RRID: AB_3670543 Conjugate: Unconjugated Form: Liquid Purification Method: Protein A purification UNIPROT ID: O75173 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.