Proteintech, 82762-11-RR, Phospho-HER2/ErbB2 (Tyr877) Recombinant monoclonal antibody

Size: 20ul / 100ul The Phospho-HER2/ErbB2 (Tyr877) (82762-11-RR) by Proteintech is a Recombinant antibody targeting Phospho-HER2/ErbB2 (Tyr877) in WB, IF/ICC, FC (Intra), ELISA applications with reactivity to human samples 82762-11-RR targets Phospho-HER2/ErbB2 (Tyr877) in WB, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human samples. Tested Applications Positive WB detected in: EGF treated A431 cells Positive IF/ICC detected in: EGF treated A431 cells Positive FC (Intra) detected in: EGF treated A431 cells Recommended dilution Western Blot (WB): WB : 1:1000-1:4000 Immunofluorescence (IF)/ICC: IF/ICC : 1:500-1:2000 Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension Background Information HER2, also known as ErbB2 and Neu, is a 185-kDa transmembrane glycoprotein that is a member of the epidermal growth factor (EGF) receptor family of receptor tyrosine kinases. Phospho-HER2/ErbB2 (Tyr877) refers to the phosphorylated form of the human epidermal growth-factor receptor 2 (HER2, ErbB2) at tyrosine 877 within its kinase domain. This phosphorylation event is catalyzed in trans by dimerization-activated HER2 itself or by Src-family kinases (SFKs) upon receptor clustering, and serves as a critical activation switch for downstream signaling cascades. ( PMID: 34107301) Specification Tested Reactivity: human Host / Isotype: Rabbit / IgG Class: Recombinant Type: Antibody Immunogen: Peptide Predict reactive species Full Name: v-erb-b2 erythroblastic leukemia viral oncogene homolog 2, neuro/glioblastoma derived oncogene homolog (avian) Calculated Molecular Weight: 1255 aa, 138 kDa Observed Molecular Weight: 185 kDa GenBank Accession Number: BC156755 Gene Symbol: HER2/ErbB2 Gene ID (NCBI): 2064 Conjugate: Unconjugated Form: Liquid Purification Method: Protein A purification UNIPROT ID: P04626 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.