Proteintech, 82773-1-RR, OGDH Recombinant monoclonal antibody

Size: 20ul / 100ul The OGDH (82773-1-RR) by Proteintech is a Recombinant antibody targeting OGDH in WB, IHC, IF-P, ELISA applications with reactivity to human, mouse, rat samples 82773-1-RR targets OGDH in WB, IHC, IF-P, ELISA applications and shows reactivity with human, mouse, rat samples. Tested Applications Positive WB detected in: HeLa cells, rat heart tissue, A549 cells, NIH/3T3 cells, C6 cells Positive IHC detected in: human intrahepatic cholangiocarcinoma tissue, mouse kidney tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive IF-P detected in: mouse kidney tissue Recommended dilution Western Blot (WB): WB : 1:2000-1:14000 Immunohistochemistry (IHC): IHC : 1:200-1:800 Immunofluorescence (IF)-P: IF-P : 1:50-1:500 Background Information OGDH(2-oxoglutarate dehydrogenase, mitochondrial) is also named as OGDC-E1and belongs to the alpha-ketoglutarate dehydrogenase family. It is one of the three enzymatic components of 2-oxoglutarate dehydrogenase complex,which catalyzes the overall conversion of 2-oxoglutarate to succinyl-CoA and CO2. This protein has 3 isoforms produced by alternative splicing. This antibody is immunized with the C-terminal and can recognize the isoform 1 and 2 with the MW of 116 kDa. Specification Tested Reactivity: human, mouse, rat Host / Isotype: Rabbit / IgG Class: Recombinant Type: Antibody Immunogen: CatNo: Ag7368 Product name: Recombinant human OGDH protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 674-1023 aa of BC004964 Sequence: SGQDVERGTFSHRHHVLHDQNVDKRTCIPMNHLWPNQAPYTVCNSSLSEYGVLGFELGFAMASPNALVLWEAQFGDFHNTAQCIIDQFICPGQAKWVRQNGIVLLLPHGMEGMGPEHSSARPERFLQMCNDDPDVLPDLKEANFDINQLYDCNWVVVNCSTPGNFFHVLRRQILLPFRKPLIIFTPKSLLRHPEARSSFDEMLPGTHFQRVIPEDGPAAQNPENVKRLLFCTGKVYYDLTRERKARDMVGQVAITRIEQLSPFPFDLLLKEVQKYPNAELAWCQEEHKNQGYYDYVKPRLRTTISRAKPVWYAGRDPAAAPATGNKKTHLTELQRLLDTAFDLDVFKNFS Predict reactive species Full Name: oxoglutarate (alpha-ketoglutarate) dehydrogenase (lipoamide) Calculated Molecular Weight: 116 kDa Observed Molecular Weight: 116 kDa GenBank Accession Number: BC004964 Gene Symbol: OGDH Gene ID (NCBI): 4967 RRID: AB_3670547 Conjugate: Unconjugated Form: Liquid Purification Method: Protein A purification UNIPROT ID: Q02218 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.