Proteintech, 82818-2-RR, EDNRB Recombinant monoclonal antibody

Size: 20ul / 100ul The EDNRB (82818-2-RR) by Proteintech is a Recombinant antibody targeting EDNRB in WB, FC (Intra), ELISA applications with reactivity to human, mouse, rat samples 82818-2-RR targets EDNRB in WB, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat samples. Tested Applications Positive WB detected in: HEK-293 cells, mouse brain tissue, mouse cerebellum tissue, rat brain tissue Positive FC (Intra) detected in: Raji cells Recommended dilution Western Blot (WB): WB : 1:1000-1:5000 Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension Background Information EDNRB (endothelin receptor type B) is a G-protein coupled heptahelical endothelin-1 (EDN1) receptor. EDN1, a known mediator of human myometrial contraction in vitro, belongs to a family of three 21-amino acid isopeptides (PMID:18497345). EDNRB is a 442-residue protein expressed post-natally in many tissues, particularly in the myenteric plexus, mucosal layer, ganglia, and blood vessels of the submucosa of the colon (PMID:11434563). Moreover, Both the EDNRA and EDNRB are expressed in vessels and can be bound by EDN1, but while EDNRA mediates a constrictive effect, EDNRB is involved in vasorelaxation and functions as a clearance receptor that scavenges circulating EDN1 (PMID:30171849). Specification Tested Reactivity: human, mouse, rat Host / Isotype: Rabbit / IgG Class: Recombinant Type: Antibody Immunogen: Peptide Predict reactive species Full Name: endothelin receptor type B Calculated Molecular Weight: 50 kDa Observed Molecular Weight: 60 kDa GenBank Accession Number: NM_000115 Gene Symbol: EDNRB Gene ID (NCBI): 1910 RRID: AB_3670561 Conjugate: Unconjugated Form: Liquid Purification Method: Protein A purification UNIPROT ID: P24530 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.