Proteintech, 82828-12-RR, Di-Methyl-Histone H3 (Lys56) Recombinant monoclonal antibody

Size: 20ul / 100ul The Di-Methyl-Histone H3 (Lys56) (82828-12-RR) by Proteintech is a Recombinant antibody targeting Di-Methyl-Histone H3 (Lys56) in WB, IF/ICC, Dot Blot, ELISA applications with reactivity to human, mouse, rat samples 82828-12-RR targets Di-Methyl-Histone H3 (Lys56) in WB, IF/ICC, Dot Blot, ELISA applications and shows reactivity with human, mouse, rat samples. Tested Applications Positive WB detected in: HeLa cells, HepG2 cells, NIH/3T3 cells, HSC-T6 cells Positive IF/ICC detected in: HepG2 cells, NIH/3T3 cells Positive Dot Blot detected in: peptide Recommended dilution Western Blot (WB): WB : 1:500-1:2000 Immunofluorescence (IF)/ICC: IF/ICC : 1:250-1:1000 DOT BLOT: DOT BLOT : 1:10-1:100 Background Information Histones, including H1/H5 (linker histones), H2, H3, and H4 (core histones), are nucleic proteins which interact with DNA to form the nucleosomes and play important roles in gene regulation and DNA replication. Histone proteins are highly post-translationally modified while Histone H3 is the most extensively modified.The NSD family histone methyltransferases, including NSD1, NSD2 and NSD3, play crucial roles in chromatin regulation and are implicated in oncogenesis (PMID: 33361816). Specification Tested Reactivity: human, mouse, rat Host / Isotype: Rabbit / IgG Class: Recombinant Type: Antibody Immunogen: Peptide Predict reactive species Full Name: histone cluster 1, H3a Observed Molecular Weight: 15-18 kDa GenBank Accession Number: BC066245 Gene Symbol: HIST1H3A Gene ID (NCBI): 8350 Conjugate: Unconjugated Form: Liquid Purification Method: Protein A purification UNIPROT ID: P68431 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.