Proteintech, 82870-7-RR, ZO-1 Recombinant monoclonal antibody

Size: 20ul / 100ul The ZO-1 (82870-7-RR) by Proteintech is a Recombinant antibody targeting ZO-1 in WB, IHC, IF/ICC, IF-P, FC (Intra), IP, ELISA applications with reactivity to human, mouse, canine samples 82870-7-RR targets ZO-1 in WB, IHC, IF/ICC, IF-P, FC (Intra), IP, ELISA applications and shows reactivity with human, mouse, canine samples. Tested Applications Positive WB detected in: HeLa cells, bEnd.3 cells, HepG2 cells, Caco-2 cells, HT-29 cells, MCF-7 cells Positive IP detected in: HEK-293 cells Positive IHC detected in: mouse colon tissue, human placenta tissue, mouse kidney tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive IF-P detected in: mouse colon tissue Positive IF/ICC detected in: MCF-7 cells, U2OS cells, MDCK cells, Caco-2 cells Positive FC (Intra) detected in: MCF-7 cells Recommended dilution Western Blot (WB): WB : 1:5000-1:50000 Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate Immunohistochemistry (IHC): IHC : 1:1000-1:4000 Immunofluorescence (IF)-P: IF-P : 1:50-1:500 Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800 Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension Background Information Tight junction (or zonula occludens) form the continuous intercellular barrier between epithelial and endothelial cells, which is required to separate tissue spaces and regulate selective movement of solutes across the epithelium and endothelium (PMID: 20066090). ZO-1 (also known as TJP1) is a peripheral membrane phosphoprotein located on the cytoplasmic face and is expressed in tight junctions of both epithelial and endothelial cells (PMID: 3528172). It binds the transmembrane proteins occludin and the claudins linking them to cytoskeletal actin (PMID: 17418867). ZO-1 belongs to a family of multidomain proteins known as the membrane-associated guanylate kinase homologs (MAGUKs). It is a pivotal tight junction protein and may be involved in signaling mechanisms regulating cell proliferation and differentiation (PMID: 22782886). Specification Tested Reactivity: human, mouse, canine Host / Isotype: Rabbit / IgG Class: Recombinant Type: Antibody Immunogen: CatNo: Ag33182 Product name: Recombinant human ZO1 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 245-428 aa of BC111712 Sequence: AKTLIERSKGKLKMVVQRDERATLLNVPDLSDSIHSANASERDDISEIQSLASDHSGRSHDRPPRRSRSRSPDQRSEPSDHSRHSPQQPSNGSLRSRDEERISKPGAVSTPVKHADDHTPKTVEEVTVERNEKQTPSLPEPKPVYAQVGQPDVDLPVSPSDGVLPNSTHEDGILRPSMKLVKFR Predict reactive species Full Name: tight junction protein 1 (zona occludens 1) Calculated Molecular Weight: 1748 aa, 195 kDa Observed Molecular Weight: 230 kDa GenBank Accession Number: BC111712 Gene Symbol: ZO-1 Gene ID (NCBI): 7082 RRID: AB_3670590 Conjugate: Unconjugated Form: Liquid Purification Method: Protein A purfication UNIPROT ID: Q07157 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.