Proteintech, 82880-1-RR, DEK Recombinant monoclonal antibody

Size: 20ul / 100ul The DEK (82880-1-RR) by Proteintech is a Recombinant antibody targeting DEK in WB, IHC, IF/ICC, FC (Intra), ELISA applications with reactivity to human, rat samples 82880-1-RR targets DEK in WB, IHC, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human, rat samples. Tested Applications Positive WB detected in: HeLa cells, HEK-293 cells, Jurkat cells, K-562 cells, HSC-T6 cells, PC-12 cells Positive IHC detected in: human skin cancer tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive IF/ICC detected in: U2OS cells, HeLa cells Positive FC (Intra) detected in: HeLa cells Recommended dilution Western Blot (WB): WB : 1:2000-1:10000 Immunohistochemistry (IHC): IHC : 1:500-1:2000 Immunofluorescence (IF)/ICC: IF/ICC : 1:500-1:2000 Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension Background Information Dek oncogene(DEK) is first recognized in a fusion with CAN nucleoporin protein, and it's a also autoantigen in patients with pquciarticular onset juvenile rheumatoid arthritis. As a site-specific binding protein, DEK may involve in transcriptional regulation and signal transdcution. Analysis by agarose gel celctrophoresis, DEK showed to reduce the efficiency with which chromation is replicated. It's one of the first components of the splicing complex, which remains associate with spliced exons dependent on prior splicing of pre-mRNA. Specification Tested Reactivity: human, rat Host / Isotype: Rabbit / IgG Class: Recombinant Type: Antibody Immunogen: CatNo: Ag8225 Product name: Recombinant human DEK protein Source: e coli. -derived, PET28a Tag: 6*His Domain: 1-375 aa of BC035259 Sequence: MSASAPAAEGEGTPTQPASEKEPEMPGPREESEEEEDEDDEEEEEEEKEKSLIVEGKREKKKVERLTMQVSSLQREPFTIAQGKGQKLCEIERIHFFLSKKKTDELRNLHKLLYNRPGTVSSLKKNVGQFSGFPFEKGSVQYKKKEEMLKKFRNAMLKSICEVLDLERSGVNSELVKRILNFLMHPKPSGKPLPKSKKTCSKGSKKERNSSGMARKAKRTKCPEILSDESSSDEDEKKNKEESSDDEDKESEEEPPKKTAKREKPKQKATSKSKKSVKSANVKKADSSTTKKNQNSSKKESESEDSSDDEPLIKKLKKPPTDEELKETIKKLLASANLEEVTMKQICKKVYENYPTYDLTERKDFIKTTVKELIS Predict reactive species Full Name: DEK oncogene Calculated Molecular Weight: 43 kDa Observed Molecular Weight: 45-50 kDa GenBank Accession Number: BC035259 Gene Symbol: DEK Gene ID (NCBI): 7913 RRID: AB_3670599 Conjugate: Unconjugated Form: Liquid Purification Method: Protein A purification UNIPROT ID: P35659 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.