Proteintech, 82895-2-RR, SLC30A1 Recombinant monoclonal antibody

Size: 20ul / 100ul The SLC30A1 (82895-2-RR) by Proteintech is a Recombinant antibody targeting SLC30A1 in WB, FC (Intra), ELISA applications with reactivity to human samples 82895-2-RR targets SLC30A1 in WB, FC (Intra), ELISA applications and shows reactivity with human samples. Tested Applications Positive WB detected in: SW480 cells, HeLa cells, HepG2 cells Positive FC (Intra) detected in: U2OS cells Recommended dilution Western Blot (WB): WB : 1:2000-1:10000 Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension Background Information Zinc transporter 1 (ZNT1), also known as SLC30A1, is the only zinc transporter predominantly located on the plasma membrane, where it plays a pivotal role exporting cytosolic zinc to the extracellular space. SLC30A1 expression is posttranslationally regulated by cellular zinc status and can be N-glycosylated on Asn299 in the extracellular loop between transmembrane domains (PMID: 15451416, 18936201). Western analysis detected a higher band at ~63 kDa than the expected molecular mass, which could be attributed to the properties of the cytosolic histidine-rich loop (PMID: 31471319). Specification Tested Reactivity: human Host / Isotype: Rabbit / IgG Class: Recombinant Type: Antibody Immunogen: CatNo: Ag18567 Product name: Recombinant human SLC30A1 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 330-507 aa of BC121015 Sequence: YTTYPLLKESALILLQTVPKQIDIRNLIKELRNVEGVEEVHELHVWQLAGSRIIATAHIKCEDPTSYMEVAKTIKDVFHNHGIHATTIQPEFASVGSKSSVVPCELACRTQCALKQCCGTLPQAPSGKDAEKTPAVSISCLELSNNLEKKPRRTKAENIPAVVIEIKNMPNKQPESSL Predict reactive species Full Name: solute carrier family 30 (zinc transporter), member 1 Calculated Molecular Weight: 507 aa, 55 kDa Observed Molecular Weight: 63~75 kDa GenBank Accession Number: BC121015 Gene Symbol: SLC30A1 Gene ID (NCBI): 7779 RRID: AB_3670624 Conjugate: Unconjugated Form: Liquid Purification Method: Protein A purification UNIPROT ID: Q9Y6M5 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.