Proteintech, 82902-1-RR, Acetyl-Histone H3 (Lys27) Recombinant monoclonal antibody

Size: 20ul / 100ul The Acetyl-Histone H3 (Lys27) (82902-1-RR) by Proteintech is a Recombinant antibody targeting Acetyl-Histone H3 (Lys27) in WB, IHC, IF/ICC, Dot Blot, ELISA, ChIP-qPCR applications with reactivity to human, mouse, rat samples 82902-1-RR targets Acetyl-Histone H3 (Lys27) in WB, IHC, IF/ICC, IP, Dot Blot, ELISA, ChIP-qPCR applications and shows reactivity with human, mouse, rat samples. Tested Applications Positive WB detected in: HeLa cells, Trichostatin A treated NIH/3T3 cells, HEK-293 cells, Jurkat cells, HSC-T6 cells, mouse kidney tissue Positive IHC detected in: mouse testis tissue, human placenta tissue, mouse liver tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive IF/ICC detected in: HeLa cells Positive Dot Blot detected in: peptide Positive ChIP-qPCR detected in: HeLa cells Recommended dilution Western Blot (WB): WB : 1:2000-1:19600 Immunohistochemistry (IHC): IHC : 1:1000-1:4000 Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800 DOT BLOT: DOT BLOT : 1:10-1:100 CHIP-QPCR: CHIP-QPCR : 1:10-1:100 Background Information Histones are small, highly basic proteins that consist of a globular domain with unstructured N- and C-terminal tails protruding from the main structure. Histone H3 is one of the five main histones that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. In addition to their role in DNA compartmentalization, histones also play crucial roles in various biologic processes, including gene expression and regulation, DNA repair, chromatin condensation, cell cycle progression, chromosome segregation, and apoptosis. The ability of histones to regulate chromatin dynamics primarily originates from various posttranslational modifications carried out by histone-modifying enzymes. Acetyl-Histone H3 (Lys27) is enhancer specific mark and plays positive role in gene expression. Specification Tested Reactivity: human, mouse, rat Cited Reactivity: human, mouse, rat Host / Isotype: Rabbit / IgG Class: Recombinant Type: Antibody Immunogen: Peptide Predict reactive species Full Name: histone cluster 1, H3a Observed Molecular Weight: 15 kDa GenBank Accession Number: BC066245 Gene Symbol: HIST1H3A Gene ID (NCBI): 8350 RRID: AB_3670631 Conjugate: Unconjugated Form: Liquid Purification Method: Protein A purification UNIPROT ID: P68431 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.