Proteintech, 82906-2-RR, AURKA Recombinant monoclonal antibody

Size: 20ul / 100ul The AURKA (82906-2-RR) by Proteintech is a Recombinant antibody targeting AURKA in WB, IF/ICC, ELISA applications with reactivity to human samples 82906-2-RR targets AURKA in WB, IF/ICC, ELISA applications and shows reactivity with human samples. Tested Applications Positive WB detected in: K-562 cells, HEK-293 cells, MCF-7 cells, HeLa cells, NCCIT cells Positive IF/ICC detected in: HepG2 cells, HeLa cells Recommended dilution Western Blot (WB): WB : 1:5000-1:50000 Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800 Background Information AURKA(Aurora kinase A), also named as STK6, STK15 or AIK, belongs to the Ser/Thr protein kinase family. This protein may play a role in cell cycle regulation during anaphase and/or telophase by being involved in microtubule formation and/or stabilization. Some recent evidence revealed that AURKA may also be implicated in tumor development and progression. AURKA is expressed highly in testis and various proliferating cells, migrating as a 46 kDa protein in SDS PAGE.Nuclear staining for AURKA is weak or nonexistent in normal tissue but strong in tumor tissue(PMID:19107951). Specification Tested Reactivity: human Host / Isotype: Rabbit / IgG Class: Recombinant Type: Antibody Immunogen: CatNo: Ag0362 Product name: Recombinant human AURKA protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 108-337 aa of BC002499 Sequence: NNPEEELASKQKNEESKKRQWALEDFEIGRPLGKGKFGNVYLAREKQSKFILALKVLFKAQLEKAGVEHQLRREVEIQSHLRHPNILRLYGYFHDATRVYLILEYAPLGTVYRELQKLSKFDEQRTATYITELANALSYCHSKRVIHRDIKPENLLLGSAGELKIADFGWSVHAPSSRRTTLCGTLDYLPPEMIEGRMHDEKVDLWSLGVLCYEFLVGKPPFEANTYQET Predict reactive species Full Name: aurora kinase A Calculated Molecular Weight: 46 kDa Observed Molecular Weight: 46-52 kDa GenBank Accession Number: BC002499 Gene Symbol: AURKA Gene ID (NCBI): 6790 RRID: AB_3670635 Conjugate: Unconjugated Form: Liquid Purification Method: Protein A purification UNIPROT ID: O14965 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.