Proteintech, 82908-1-RR, SEC24A Recombinant monoclonal antibody

Size: 20ul / 100ul The SEC24A (82908-1-RR) by Proteintech is a Recombinant antibody targeting SEC24A in WB, IF/ICC, FC (Intra), ELISA applications with reactivity to human samples 82908-1-RR targets SEC24A in WB, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human samples. Tested Applications Positive WB detected in: U-87 MG cells, HEK-293 cells, HeLa cells, HepG2 cells Positive IF/ICC detected in: HeLa cells Positive FC (Intra) detected in: HepG2 cells Recommended dilution Western Blot (WB): WB : 1:1000-1:5000 Immunofluorescence (IF)/ICC: IF/ICC : 1:125-1:500 Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension Background Information COPII-coated vesicles mediate anterograde transport from the ER to the Golgi apparatus. COPII is composed of three parts: two coat protein complexes (Sec23/24 complex and Sec13/31 complex) and one small GTP-binding protein, Sar1 (PMID: 9568718). Sec24 subunit of the coat complex has been implicated in cargo recognition (PMID: 17316621). In mammal cells, there are four Sec24 isoforms, termed Sec24A, Sec24B, Sec24C, and Sec24D (PMID: 10329445). This antibody is specific to SEC24A. Specification Tested Reactivity: human Host / Isotype: Rabbit / IgG Class: Recombinant Type: Antibody Immunogen: CatNo: Ag8723 Product name: Recombinant human SEC24A protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 264-613 aa of BC019341 Sequence: DEIEGGGLLATPQLTNKNPKMSRSVGYSYPSLPPGYQNITPPGATGVPPSSLNYPSGPQAFTQTPLGANHLTTSMSGLSLQPEGLRVVNLLQERNMLPSTPLKPPVPNLHEDIQKLNCNPELFRCTLTSIPQMQALLNKAKLPLGLLLHPFKDLVQLPVVTSSTIVRCRSCRTYINPFVSFLDQRRWKCNLCYRVNDVPEEFLYNPLTRVYGEPHRRPEVQNATIEFMAPSEYMLRPPQPPVYLFVFDVSHNAVETGYLNSVCQSLLDNLDLLPGNTRTKIGFITFDSTIHFYGLQESLSQPQMLIVSDIEDVFIPMPENLLVNLNESKESVIGVSSEETLITCLEIAMR Predict reactive species Full Name: SEC24 family, member A (S. cerevisiae) Calculated Molecular Weight: 613 aa, 66 kDa Observed Molecular Weight: 120 kDa GenBank Accession Number: BC019341 Gene Symbol: SEC24A Gene ID (NCBI): 10802 RRID: AB_3670637 Conjugate: Unconjugated Form: Liquid Purification Method: Protein A purification UNIPROT ID: O95486 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.