Proteintech, 82978-1-RR, RAN Recombinant monoclonal antibody

Size: 20ul / 100ul The RAN (82978-1-RR) by Proteintech is a Recombinant antibody targeting RAN in WB, IHC, IF/ICC, FC (Intra), ELISA applications with reactivity to human, mouse, rat samples 82978-1-RR targets RAN in WB, IHC, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat samples. Tested Applications Positive WB detected in: HeLa cells, HEK-293 cells, A375 cells, Jurkat cells, NIH/3T3 cells, C6 cells Positive IHC detected in: human colon tissue, mouse kidney tissue, rat kidney tissue, human placenta tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive IF/ICC detected in: HeLa cells Positive FC (Intra) detected in: HeLa cells, A431 cells Recommended dilution Western Blot (WB): WB : 1:1000-1:5000 Immunohistochemistry (IHC): IHC : 1:250-1:1000 Immunofluorescence (IF)/ICC: IF/ICC : 1:125-1:500 Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension Background Information RAN is also named as ARA24, Ras-like protein TC4 and GTPase Ran, and belongs to the small GTPase superfamily family and the Ran family. GTPase Ran switches between a cytoplasmic GDP- and a nuclear GTP-bound state by nucleotide exchange and GTP hydrolysis (PMID:7819259, PMID:32528950). RAN (GTP-bound form) triggers microtubule assembly at mitotic chromosomes and is required for normal mitotic spindle assembly and chromosome segregation (PMID:10408446, PMID:29040603, PMID:32528950). Specification Tested Reactivity: human, mouse, rat Host / Isotype: Rabbit / IgG Class: Recombinant Type: Antibody Immunogen: CatNo: Ag0748 Product name: Recombinant human RAN protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 1-216 aa of BC004272 Sequence: MAAQGEPQVQFKLVLVGDGGTGKTTFVKRHLTGEFEKKYVATLGVEVHPLVFHTNRGPIKFNVWDTAGQEKFGGLRDGYYIQAQCAIIMFDVTSRVTYKNVPNWHRDLVRVCENIPIVLCGNKVDIKDRKVKAKSIVFHRKKNLQYYDISAKSNYNFEKPFLWLARKLIGDPNLEFVAMPALAPPEVVMDPALAAQYEHDLEVAQTTALPDEDDDL Predict reactive species Full Name: RAN, member RAS oncogene family Calculated Molecular Weight: 25 kDa Observed Molecular Weight: 24 kDa GenBank Accession Number: BC004272 Gene Symbol: RAN Gene ID (NCBI): 5901 RRID: AB_3670726 Conjugate: Unconjugated Form: Liquid Purification Method: Protein A purification UNIPROT ID: P62826 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.