Proteintech, 83002-4-RR, IFNAR1 Recombinant monoclonal antibody

Size: 20ul / 100ul The IFNAR1 (83002-4-RR) by Proteintech is a Recombinant antibody targeting IFNAR1 in WB, FC, ELISA applications with reactivity to human samples 83002-4-RR targets IFNAR1 in WB, FC, ELISA applications and shows reactivity with human samples. Tested Applications Positive WB detected in: K-562 cells, HeLa cells, HepG2 cells, HEK-293 cells, A431 cells, Jurkat cells Positive FC detected in: Jurkat cells, K562 Recommended dilution Western Blot (WB): WB : 1:5000-1:50000 Flow Cytometry (FC): FC : 0.25 ug per 10^6 cells in a 100 µl suspension Background Information Interferon alpha and beta receptor subunit 1 (IFNAR1) is a type I membrane protein that associates with IFNAR2 to form the receptor for type I interferons, including interferons-alpha, -beta, and -lambda. Binding and activation of the receptor stimulates Janus protein kinases, which in turn phosphorylate several proteins, including STAT1 and STAT2, and then increase transcription of the IFN-induced genes whose products exert antiviral, immunomodulatory, and antiproliferative effects. Specification Tested Reactivity: human Host / Isotype: Rabbit / IgG Class: Recombinant Type: Antibody Immunogen: CatNo: Eg0156 Product name: Recombinant Human IFNAR1 protein (Myc Tag, His Tag) Source: mammalian cells -derived, pHZ-KIsec Tag: Myc & 6*His Domain: 28-436 aa of BC021825 Sequence: KNLKSPQKVEVDIIDDNFILRWNRSDESVGNVTFSFDYQKTGMDNWIKLSGCQNITSTKCNFSSLKLNVYEEIKLRIRAEKENTSSWYEVDSFTPFRKAQIGPPEVHLEAEDKAIVIHISPGTKDSVMWALDGLSFTYSLVIWKNSSGVEERIENIYSRHKIYKLSPETTYCLKVKAALLTSWKIGVYSPVHCIKTTVENELPPPENIEVSVQNQNYVLKWDYTYANMTFQVQWLHAFLKRNPGNHLYKWKQIPDCENVKTTQCVFPQNVFQKGIYLLRVQASDGNNTSFWSEEIKFDTEIQAFLLPPVFNIRSLSDSFHIYIGAPKQSGNTPVIQDYPLIYEIIFWENTSNAERKIIEKKTDVTVPNLKPLTVYCVKARAHTMDEKLNKSSVFSDAVCEKTKPGNTSK Predict reactive species Full Name: interferon (alpha, beta and omega) receptor 1 Calculated Molecular Weight: 557 aa, 64 kDa Observed Molecular Weight: 80-140 kDa GenBank Accession Number: BC021825 Gene Symbol: IFNAR1 Gene ID (NCBI): 3454 RRID: AB_3670748 Conjugate: Unconjugated Form: Liquid Purification Method: Protein A purfication UNIPROT ID: P17181 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.