Proteintech, 83027-4-RR, SCN4B Recombinant monoclonal antibody

Size: 20ul / 100ul The SCN4B (83027-4-RR) by Proteintech is a Recombinant antibody targeting SCN4B in WB, IHC, FC (Intra), ELISA applications with reactivity to human, mouse, rat samples 83027-4-RR targets SCN4B in WB, IHC, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat samples. Tested Applications Positive WB detected in: U-87 MG cells, HeLa cells, Ramos cells, C6 cells, mouse brain tissue, human heart tissue Positive IHC detected in: rat dorsal root ganglion tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive FC (Intra) detected in: HeLa cells Recommended dilution Western Blot (WB): WB : 1:1000-1:4000 Immunohistochemistry (IHC): IHC : 1:500-1:2000 Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension Background Information Mammalian sodium channels are heterotrimers, composed of a central, pore-forming alpha subunit and two auxiliary beta subunits (PMID: 11486343). SCN4B (sodium channel subunit beta-4) is one of several sodium channel beta subunits, which modulate channel gating kinetics. SCN4B has an N-terminal signal peptide, a large extracellular domain predicted to form an Ig-like fold, a single transmembrane alpha helix, and a short cytoplasmic C terminus. Defects in SCN4B gene are a cause of long QT syndrome type 10 (LQT10). Specification Tested Reactivity: human, mouse, rat Host / Isotype: Rabbit / IgG Class: Recombinant Type: Antibody Immunogen: CatNo: Ag34123 Product name: Recombinant human SCN4B protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 31-162 aa of NM_174934.3 Sequence: LEVSVGKATDIYAVNGTEILLPCTFSSCFGFEDLHFRWTYNSSDAFKILIEGTVKNEKSDPKVTLKDDDRITLVGSTKEKMNNISIVLRDLEFSDTGKYTCHVKNPKENNLQHHATIFLQVVDRLEEVDNTV Predict reactive species Full Name: sodium channel, voltage-gated, type IV, beta Calculated Molecular Weight: 25 kDa Observed Molecular Weight: 37 kDa GenBank Accession Number: NM_174934.3 Gene Symbol: SCN4B Gene ID (NCBI): 6330 RRID: AB_3670767 Conjugate: Unconjugated Form: Liquid Purification Method: Protein A purification UNIPROT ID: Q8IWT1 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.