Proteintech, 83042-3-RR, MTA3 Recombinant monoclonal antibody

Size: 20ul / 100ul The MTA3 (83042-3-RR) by Proteintech is a Recombinant antibody targeting MTA3 in WB, IF/ICC, FC (Intra), ELISA applications with reactivity to human, mouse samples 83042-3-RR targets MTA3 in WB, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human, mouse samples. Tested Applications Positive WB detected in: Jurkat cells, MCF-7 cells, A375 cells, NIH/3T3 cells, C2C12 cells, Raji cells Positive IF/ICC detected in: U-251 cells Positive FC (Intra) detected in: U-251 cells Recommended dilution Western Blot (WB): WB : 1:5000-1:50000 Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500 Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension Background Information The metastasis-associated (MTA) family contains three members, MTA1, MTA2 and MTA3 (PMID:12167865).Metastasis-associated protein 3 (MTA3) is a cell-type specific component of the Mi-2-NURD transcriptional co-repressor complex. It has a role in maintenance of the normal epithelial architecture through the repression of SNAI1 transcription in a histone deacetylase-dependent manner, and thus the regulation of E-cadherin levels. Specification Tested Reactivity: human, mouse Host / Isotype: Rabbit / IgG Class: Recombinant Type: Antibody Immunogen: CatNo: Ag6400 Product name: Recombinant human MTA3 protein Source: e coli. -derived, PKG Tag: GST Domain: 1-343 aa of BC053631 Sequence: MAANMYRVGDYVYFENSSSNPYLIRRIEELNKTASGNVEAKVVCFYRRRDISNTLIMLADKHAKEIEEESETTVEADLTDKQKHQLKHRELFLSRQYESLPATHIRGKCSVALLNETESVLSYLDKEDTFFYSLVYDPSLKTLLADKGEIRVGPRYQADIPEMLLEGESDEREQSKLEVKVWDPNSPLTDRQIDQFLVVARAVGTFARALDCSSSVRQPSLHMSAAAASRDITLFHAMDTLYRHSYDLSSAISVLVPLGGPVLCRDEMEEWSASEASLFEEALEKYGKDFNDIRQDFLPWKSLTSIIEYYYMWKTTDRYVQQKRLKAAEAESKLKQVYIPTYS Predict reactive species Full Name: metastasis associated 1 family, member 3 Calculated Molecular Weight: 68 kDa Observed Molecular Weight: 62 kDa, 68 kDa GenBank Accession Number: BC053631 Gene Symbol: MTA3 Gene ID (NCBI): 57504 RRID: AB_3670772 Conjugate: Unconjugated Form: Liquid Purification Method: Protein A purfication UNIPROT ID: Q9BTC8 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.