Proteintech, 83177-5-RR, TPR Recombinant monoclonal antibody

Size: 20ul / 100ul The TPR (83177-5-RR) by Proteintech is a Recombinant antibody targeting TPR in WB, IHC, IF/ICC, ELISA applications with reactivity to human samples 83177-5-RR targets TPR in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples. Tested Applications Positive WB detected in: HeLa cells, HEK-293 cells Positive IHC detected in: mouse testis tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive IF/ICC detected in: HeLa cells Recommended dilution Western Blot (WB): WB : 1:500-1:2000 Immunohistochemistry (IHC): IHC : 1:50-1:500 Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500 Background Information Nucleoporin TPR was originally identified as the oncogenic activator of the met, raf, and trk protooncogenes. TPR is a large 270 kDa protein with a bipartite structure consisting of a ~1600-residue a-helical coiled-coil N-terminal domain and a highly acidic non-coiled ~800 amino acid carboxy terminus (PMID: 22253824). TPR is localized exclusively to intranuclear filaments associated with the nucleoplasmic side of the NPC, by directly binding to Nup153 (PMID: 12802065). Specification Tested Reactivity: human Host / Isotype: Rabbit / IgG Class: Recombinant Type: Antibody Immunogen: Peptide Predict reactive species Full Name: translocated promoter region (to activated MET oncogene) Calculated Molecular Weight: 267 kDa Observed Molecular Weight: ~270 kDa GenBank Accession Number: NM_003292 Gene Symbol: TPR Gene ID (NCBI): 7175 RRID: AB_3670870 Conjugate: Unconjugated Form: Liquid Purification Method: Protein A purfication UNIPROT ID: P12270 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.