Proteintech, 83185-5-RR, Hemoglobin Alpha Recombinant monoclonal antibody

Size: 20ul / 100ul The Hemoglobin Alpha (83185-5-RR) by Proteintech is a Recombinant antibody targeting Hemoglobin Alpha in WB, FC (Intra), ELISA applications with reactivity to human samples 83185-5-RR targets Hemoglobin Alpha in WB, FC (Intra), ELISA applications and shows reactivity with human samples. Tested Applications Positive WB detected in: human placenta tissue Positive FC (Intra) detected in: K-562 cells, TF-1 cells Recommended dilution Western Blot (WB): WB : 1:1000-1:5000 Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension Background Information The hemoglobin molecule is a tetramer consisting of two alpha- and two beta-globin-like chains. HBA1 (hemoglobin alpha chain) protein is a alpha-type chain of hemoglobin encoded by two independent genes (HBA1 and HBA2) whose coding sequences are identical. Two alpha chains coupled with two beta chains constitute the adult hemoglobin (HbA or α2β2). HBA1 is also the component of fetal hemoglobin (HbF or α2γ2). This antibody detects a major band around 14-16 kDa in the western blot analysis of heart tissue and K-562 cells. A higher band around 26-27 kDa can also be observed occasionally, which may represents the dimer form of HBA1 (PMID: 20836851). This antibody may cross-react with other hemoglobin chains. Specification Tested Reactivity: human Host / Isotype: Rabbit / IgG Class: Recombinant Type: Antibody Immunogen: CatNo: Ag6040 Product name: Recombinant human HBA1 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 1-142 aa of BC050661 Sequence: MVLSPADKTNVKAAWGKVGAHAGEYGAEALERMFLSFPTTKTYFPHFDLSHGSAQVKGHGKKVADALTNAVAHVDDMPNALSALSDLHAHKLRVDPVNFKLLSHCLLVTLAAHLPAEFTPAVHASLDKFLASVSTVLTSKYR Predict reactive species Full Name: hemoglobin, alpha 1 Calculated Molecular Weight: 15 kDa Observed Molecular Weight: 14-16 kDa, 27 kDa GenBank Accession Number: BC050661 Gene Symbol: HBA1 Gene ID (NCBI): 3039 RRID: AB_3670874 Conjugate: Unconjugated Form: Liquid Purification Method: Protein A purfication UNIPROT ID: P69905 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.