Proteintech, 83210-2-RR, LAMA3 Recombinant monoclonal antibody

Size: 20ul / 100ul The LAMA3 (83210-2-RR) by Proteintech is a Recombinant antibody targeting LAMA3 in WB, IHC, ELISA applications with reactivity to human samples 83210-2-RR targets LAMA3 in WB, IHC, ELISA applications and shows reactivity with human samples. Tested Applications Positive WB detected in: HeLa cells, HepG2 cells, NCI-H1299 cells, HaCaT cells, A549 cells Positive IHC detected in: human intrahepatic cholangiocarcinoma tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Recommended dilution Western Blot (WB): WB : 1:5000-1:50000 Immunohistochemistry (IHC): IHC : 1:500-1:2000 Background Information Laminin subunit alpha 3 (LAMA3) expression is mediated by an epithelial enhancer via a synergistic effect of AP-1 binding sites.10 LAMA3 has been implicated in the invasion and progression of tumor cells and encodes for the α3 chain of laminin-5 (α3, β3, γ2, 332). Its dysfunction manifests as a blistering skin disease similar to junctional epidermolysis bullosa in humans. (PMID: 31557618) Specification Tested Reactivity: human Cited Reactivity: human Host / Isotype: Rabbit / IgG Class: Recombinant Type: Antibody Immunogen: Peptide Predict reactive species Full Name: laminin, alpha 3 Calculated Molecular Weight: 367 kDa Observed Molecular Weight: 260 kDa GenBank Accession Number: NM_000227 Gene Symbol: LAMA3 Gene ID (NCBI): 3909 RRID: AB_3670895 Conjugate: Unconjugated Form: Liquid Purification Method: Protein A purification UNIPROT ID: Q16787 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.