Proteintech, 83216-3-RR, NDUFB8 Recombinant monoclonal antibody

Size: 20ul / 100ul The NDUFB8 (83216-3-RR) by Proteintech is a Recombinant antibody targeting NDUFB8 in WB, IHC, IF/ICC, FC (Intra), IP, ELISA applications with reactivity to human, mouse, rat samples 83216-3-RR targets NDUFB8 in WB, IHC, IF/ICC, FC (Intra), IP, ELISA applications and shows reactivity with human, mouse, rat samples. Tested Applications Positive WB detected in: HepG2 cells, A549 cells, mouse liver tissue, mouse brain tissue, rat brain tissue, HeLa cells, Jurkat cells Positive IP detected in: HepG2 cells Positive IHC detected in: human stomach cancer tissue, human intrahepatic cholangiocarcinoma tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive IF/ICC detected in: HepG2 cells Positive FC (Intra) detected in: HeLa cells Recommended dilution Western Blot (WB): WB : 1:5000-1:50000 Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate Immunohistochemistry (IHC): IHC : 1:200-1:800 Immunofluorescence (IF)/ICC: IF/ICC : 1:125-1:500 Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension Specification Tested Reactivity: human, mouse, rat Host / Isotype: Rabbit / IgG Class: Recombinant Type: Antibody Immunogen: CatNo: Ag6495 Product name: Recombinant human NDUFB8 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 1-186 aa of BC000466 Sequence: MAVARAGVLGVQWLQRASRNVMPLGARTASHMTKDMFPGPYPRTPEERAAAAKKYNMRVEDYEPYPDDGMGYGDYPKLPDRSQHERDPWYSWDQPGLRLNWGEPMHWHLDMYNRNRVDTSPTPVSWHVMCMQLFGFLAFMIFMCWVGDVYPVYQPVGPKQYPYNNLYLERGGDPSKEPERVVHYEI Predict reactive species Full Name: NADH dehydrogenase (ubiquinone) 1 beta subcomplex, 8, 19kDa Calculated Molecular Weight: 22 kDa Observed Molecular Weight: 19-22 kDa GenBank Accession Number: BC000466 Gene Symbol: NDUFB8 Gene ID (NCBI): 4714 RRID: AB_3670901 Conjugate: Unconjugated Form: Liquid Purification Method: Protein A purfication UNIPROT ID: O95169 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.