Proteintech, 83331-1-RR, Phospho-SYK (Tyr323) Recombinant monoclonal antibody

Size: 20ul / 100ul The Phospho-SYK (Tyr323) (83331-1-RR) by Proteintech is a Recombinant antibody targeting Phospho-SYK (Tyr323) in WB, IF/ICC, ELISA applications with reactivity to Human samples 83331-1-RR targets Phospho-SYK (Tyr323) in WB, IF/ICC, ELISA applications and shows reactivity with Human samples. Tested Applications Positive WB detected in: pervanadate treated Ramos cells Positive IF/ICC detected in: pervanadate treated Ramos cells Recommended dilution Western Blot (WB): WB : 1:5000-1:50000 Immunofluorescence (IF)/ICC: IF/ICC : 1:125-1:500 Background Information SYK(spleen tyrosine kinase) is a key regulator of signal transduction events,apoptosis and orderly cell cycle progression in B-lineage lymphoid cells. The study found that in human platelets, inhibition of PKC leads to Syk hyperphosphorylation on residues Tyr-525/526 whereas Tyr-323 and Tyr-352 phosphorylations are unaffected. PKC negatively regulates Syk activity, as evidenced by hyperphosphorylation of downstream targets, LAT and PLCγ2 upon PKC inhibition. (PMID: 23960082) Specification Tested Reactivity: Human Host / Isotype: Rabbit / IgG Class: Recombinant Type: Antibody Immunogen: Peptide Predict reactive species Full Name: spleen tyrosine kinase Calculated Molecular Weight: 72 kDa Observed Molecular Weight: 70 kDa GenBank Accession Number: BC001645 Gene Symbol: SYK Gene ID (NCBI): 6850 ENSEMBL Gene ID: ENSG00000165025 RRID: AB_3670995 Conjugate: Unconjugated Form: Liquid Purification Method: Protein A purfication UNIPROT ID: P43405 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.