Proteintech, 83354-3-RR, CNKSR2 Recombinant monoclonal antibody

Size: 20ul / 100ul The CNKSR2 (83354-3-RR) by Proteintech is a Recombinant antibody targeting CNKSR2 in WB, FC (Intra), ELISA applications with reactivity to human, mouse samples 83354-3-RR targets CNKSR2 in WB, FC (Intra), ELISA applications and shows reactivity with human, mouse samples. Tested Applications Positive WB detected in: A549 cells, Jurkat cells, MDA-MB-231 cells, U-251 cells, mouse brain tissue, U-87 MG cellls, SK-BR-3 cells Positive FC (Intra) detected in: Jurkat cells Recommended dilution Western Blot (WB): WB : 1:2000-1:10000 Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension Background Information CNKSR2 is a multidomain protein that functions as a scaffold protein to mediate the mitogen-activated protein kinase pathways downstream from Ras. This gene product is induced by vitamin D and inhibits apoptosis in certain cancer cells. It may also play a role in ternary complex assembly of synaptic proteins at the postsynaptic membrane and coupling of signal transduction to membrane/cytoskeletal remodeling. Specification Tested Reactivity: human, mouse Host / Isotype: Rabbit / IgG Class: Recombinant Type: Antibody Immunogen: CatNo: Ag21174 Product name: Recombinant human CNKSR2 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 400-558 aa of BC126121 Sequence: RKRFNIVEEDTVLYCYEYEKGRSSSQGRRESTPTYGKLRPISMPVEYNWVGDYEDPNKMKRDSRRENSLLRYMSNEKIAQEEYMFQRNSKKDTGKKSKKKGDKSNSPTHYSLLPSLQMDALRQDIMGTPVPETTLYHTFQQSSLQHKSKKKNKGPIAGK Predict reactive species Full Name: connector enhancer of kinase suppressor of Ras 2 Calculated Molecular Weight: 1034 aa, 118 kDa Observed Molecular Weight: 118 kDa, 102 kDa GenBank Accession Number: BC126121 Gene Symbol: CNKSR2 Gene ID (NCBI): 22866 RRID: AB_3671011 Conjugate: Unconjugated Form: Liquid Purification Method: Protein A purification UNIPROT ID: Q8WXI2 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.