Proteintech, 83407-7-RR, FEZ2 Recombinant monoclonal antibody

Size: 20ul / 100ul The FEZ2 (83407-7-RR) by Proteintech is a Recombinant antibody targeting FEZ2 in WB, IHC, FC (Intra), ELISA applications with reactivity to human samples 83407-7-RR targets FEZ2 in WB, IHC, FC (Intra), ELISA applications and shows reactivity with human samples. Tested Applications Positive WB detected in: HeLa cells, Jurkat cells, HepG2 cells, A375 cells Positive IHC detected in: human ovary cancer tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive FC (Intra) detected in: A431 cells Recommended dilution Western Blot (WB): WB : 1:5000-1:50000 Immunohistochemistry (IHC): IHC : 1:50-1:500 Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension Background Information FEZ2 is an ortholog of the C. elegans unc-76 gene, which is necessary for normal axonal bundling and elongation within axon bundles. Specification Tested Reactivity: human Host / Isotype: Rabbit / IgG Class: Recombinant Type: Antibody Immunogen: CatNo: Ag15370 Product name: Recombinant human FEZ2 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 1-160 aa of BC110386 Sequence: MAADGDWQDFYEFQEPARSLLDQENCNASPEPGAEAGAGAGGGADGFPALACSLEEKLSLCFRPSDPGAEPPRTAVRPITERSLLQGDEIWNALTDNYGNVMPVDWKSSHTRTLHLLTLNLSEKGVSDSLLFDTSDDEELREQLDMHSIIVSCVNDEPLF Predict reactive species Full Name: fasciculation and elongation protein zeta 2 (zygin II) Calculated Molecular Weight: 353 aa, 40 kDa Observed Molecular Weight: ~50 kDa GenBank Accession Number: BC110386 Gene Symbol: FEZ2 Gene ID (NCBI): 9637 RRID: AB_3671055 Conjugate: Unconjugated Form: Liquid Purification Method: Protein A purfication UNIPROT ID: Q9UHY8 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.