Proteintech, 83451-4-RR, CD5 Recombinant monoclonal antibody

Size: 20ul / 100ul The CD5 (83451-4-RR) by Proteintech is a Recombinant antibody targeting CD5 in WB, IHC, IF-P, ELISA applications with reactivity to mouse samples 83451-4-RR targets CD5 in WB, IHC, IF-P, ELISA applications and shows reactivity with mouse samples. Tested Applications Positive WB detected in: EL-4 cells, mouse thymus tissue Positive IHC detected in: mouse spleen tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive IF-P detected in: mouse spleen tissue Recommended dilution Western Blot (WB): WB : 1:500-1:2000 Immunohistochemistry (IHC): IHC : 1:500-1:2000 Immunofluorescence (IF)-P: IF-P : 1:200-1:800 Background Information CD5 is a type I transmembrane glycoprotein of the scavenger receptor cysteine-rich family (PMID: 12403363). CD5 is expressed on a majority of thymocytes, mature T cells, B cell subsets, and peripheral blood dendritic cells (PMID: 9858516; 6156984; 10379049; 1384337). CD5 may act as a receptor in regulating T-cell proliferation. It functions as a negative regulator of TCR signaling during thymocyte development (PMID: 7542801). Specification Tested Reactivity: mouse Host / Isotype: Rabbit / IgG Class: Recombinant Type: Antibody Immunogen: Recombinant protein Predict reactive species Full Name: CD5 antigen Calculated Molecular Weight: 54 kDa Observed Molecular Weight: 55 kDa, 67 kDa GenBank Accession Number: NM_007650.3 Gene Symbol: Cd5 Gene ID (NCBI): 12507 RRID: AB_3671088 Conjugate: Unconjugated Form: Liquid Purification Method: Protein A purfication UNIPROT ID: P13379 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.