Proteintech, 83480-5-RR, MAGEA3 Recombinant monoclonal antibody

Size: 20ul / 100ul The MAGEA3 (83480-5-RR) by Proteintech is a Recombinant antibody targeting MAGEA3 in WB, IF/ICC, FC (Intra), ELISA applications with reactivity to human samples 83480-5-RR targets MAGEA3 in WB, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human samples. Tested Applications Positive WB detected in: A375 cells, Jurkat cells, SGC-7901 cells, NCI-H1299 cells Positive IF/ICC detected in: A549 cells Positive FC (Intra) detected in: HeLa cells, Jurkat cells Recommended dilution Western Blot (WB): WB : 1:5000-1:50000 Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800 Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension Background Information MAGEA3 is a member of the MAGEA family, which belongs to chromosome X-clustered cancer/testis antigens. MAGEA3 is expressed in various human cancers including melanoma, brain, breast, lung, thyroid, colorectal, gastric, pancreatic and ovarian cancer. The aberrant expression of MAGEA3 is associated with poor clinical prognosis in many tumor types. The antibody is specific to MAGEA3. Specification Tested Reactivity: human Host / Isotype: Rabbit / IgG Class: Recombinant Type: Antibody Immunogen: CatNo: Ag0265 Product name: Recombinant human MAGEA3 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 1-229 aa of BC000340 Sequence: MPLEQRSQHCKPEEGLEARGEALGLVGAQAPATEEQEAASSSSTLVEVTLGEVPAAESPDPPQSPQGASSLPTTMNYPLWSQSYEDSSNQEEEGPSTFPDLESEFQAALSRKVAELVHFLLLKYRAREPVTKAEMLGSVVGNWQYFFPVIFSKASSSLQLVFGIELMEVDPIGHLYIFATCLGLSYDGLLGDNQIMPKAGLLIIVLAIIAREGDCAPEEKIWEELSVLE Predict reactive species Full Name: melanoma antigen family A, 3 Calculated Molecular Weight: 314 aa, 35 kDa Observed Molecular Weight: 45 kDa GenBank Accession Number: BC000340 Gene Symbol: MAGEA3 Gene ID (NCBI): 4102 RRID: AB_3671108 Conjugate: Unconjugated Form: Liquid Purification Method: Protein A purification UNIPROT ID: P43357 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.