Proteintech, 83515-4-RR, MRPL40 Recombinant monoclonal antibody

Size: 20ul / 100ul The MRPL40 (83515-4-RR) by Proteintech is a Recombinant antibody targeting MRPL40 in WB, IHC, IF/ICC, ELISA applications with reactivity to human samples 83515-4-RR targets MRPL40 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples. Tested Applications Positive WB detected in: A549 cells, HeLa cells, K-562 cells, LNCaP cells, Jurkat cells, COLO 320 cells Positive IHC detected in: human intrahepatic cholangiocarcinoma tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive IF/ICC detected in: HeLa cells Recommended dilution Western Blot (WB): WB : 1:5000-1:50000 Immunohistochemistry (IHC): IHC : 1:50-1:500 Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800 Background Information MRPL40, also known as NLVCF and URIM, belongs to the mitochondrion-specific ribosomal protein mL40 family. MRPL40 encodes the mitochondrial ribosomal protein L40 for the larger 39S subunit of mitochondrial ribosomes. MRPL40 is widely expressed in human organs and tissues, including the heart, brain, placenta, lung, liver, skeletal muscle, kidney, pancreas, spleen, thymus, prostate, ovary, small intestine, colon, peripheral blood lymphocyte (PBL), and testis. MRPL40 is also expressed in human fetuses and mouse embryos, suggesting its important role in fetal development (PMID: 36891938). Specification Tested Reactivity: human Host / Isotype: Rabbit / IgG Class: Recombinant Type: Antibody Immunogen: CatNo: Ag10341 Product name: Recombinant human MRPL40 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 1-206 aa of BC009707 Sequence: MTASVLRSISLALRPTSGLLGTWQTQLRETHQRASLLSFWELIPMRSEPLRKKKKVDPKKDQEAKERLKRKIRKLEKATQELIPIEDFITPLKFLDKARERPQVELTFEETERRALLLKKWSLYKQQERKMERDTIRAMLEAQQEALEELQLESPKLHAEAIKRDPNLFPFEKEGPHYTPPIPNYQPPEGRYNDITKVYTQVEFKR Predict reactive species Full Name: mitochondrial ribosomal protein L40 Calculated Molecular Weight: 206 aa, 24 kDa Observed Molecular Weight: 18-24 kDa GenBank Accession Number: BC009707 Gene Symbol: MRPL40 Gene ID (NCBI): 64976 RRID: AB_3671140 Conjugate: Unconjugated Form: Liquid Purification Method: Protein A purfication UNIPROT ID: Q9NQ50 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.