Proteintech, 83598-4-RR, Flotillin 1 Recombinant monoclonal antibody

Size: 20ul / 100ul The Flotillin 1 (83598-4-RR) by Proteintech is a Recombinant antibody targeting Flotillin 1 in WB, IHC, IF/ICC, IP, ELISA applications with reactivity to human, mouse, rat samples 83598-4-RR targets Flotillin 1 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples. Tested Applications Positive WB detected in: U-87 MG cells, U-251 cells, LNCaP cells, Raji cells, mouse heart tissue, mouse brain tissue, rat brain tissue Positive IP detected in: mouse brain tissue Positive IHC detected in: human stomach cancer tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive IF/ICC detected in: HeLa cells Recommended dilution Western Blot (WB): WB : 1:5000-1:50000 Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate Immunohistochemistry (IHC): IHC : 1:200-1:800 Immunofluorescence (IF)/ICC: IF/ICC : 1:250-1:1000 Background Information Flotillins are membrane-associated proteins that contain two homologous isoforms, flotillin 1 (FLOT1) and flotillin 2 (FLOT2). They are highly conserved and widely expressed proteins commonly used as lipid raft markers. Flotillins are involved in various signaling pathways, cell adhesion, membrane trafficking and axonal growth. Specification Tested Reactivity: human, mouse, rat Host / Isotype: Rabbit / IgG Class: Recombinant Type: Antibody Immunogen: CatNo: Ag7902 Product name: Recombinant human Flotillin 1 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 80-427 aa of BC001146 Sequence: MLAAACQMFLGKTEAEIAHIALETLEGHQRAIMAHMTVEEIYKDRQKFSEQVFKVASSDLVNMGISVVSYTLKDIHDDQDYLHSLGKARTAQVQKDARIGEAEAKRDAGIREAKAKQEKVSAQYLSEIEMAKAQRDYELKKAAYDIEVNTRRAQADLAYQLQVAKTKQQIEEQRVQVQVVERAQQVAVQEQEIARREKELEARVRKPAEAERYKLERLAEAEKSQLIMQAEAEAASVRMRGEAEAFAIGARARAEAEQMAKKAEAFQLYQEAAQLDMLLEKLPQVAEEISGPLTSANKITLVSSGSGTMGAAKVTGEVLDILTRLPESVERLTGVSISQVNHKPLRTA Predict reactive species Full Name: flotillin 1 Calculated Molecular Weight: 47 kDa Observed Molecular Weight: 47 kDa GenBank Accession Number: BC001146 Gene Symbol: Flotillin 1 Gene ID (NCBI): 10211 RRID: AB_3671213 Conjugate: Unconjugated Form: Liquid Purification Method: Protein A purfication UNIPROT ID: O75955 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.