Proteintech, 83646-6-RR, SWAP70 Recombinant monoclonal antibody

Size: 20ul / 100ul The SWAP70 (83646-6-RR) by Proteintech is a Recombinant antibody targeting SWAP70 in WB, IHC, FC (Intra), ELISA applications with reactivity to human, mouse samples 83646-6-RR targets SWAP70 in WB, IHC, FC (Intra), ELISA applications and shows reactivity with human, mouse samples. Tested Applications Positive WB detected in: HeLa cells, HepG2 cells, NIH/3T3 cells, Daudi cells, NIC-H1299 cells, HEK-293 cells, A549 cellls Positive IHC detected in: mouse spleen tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive FC (Intra) detected in: SK-N-SH cells, U2OS cells Recommended dilution Western Blot (WB): WB : 1:5000-1:50000 Immunohistochemistry (IHC): IHC : 1:250-1:1000 Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension Background Information SWAP70 (switching B cell complex subunit), also known as HSPC321. It is expected to be located in cell membrane, cytoplasm and nucleus, the protein is mainly expressed in spleen, and fairly abundant in kidney, lung and liver. Also found in monocytes, macrophages and mature B-cells (PMID: 10681448). The calculated molecular weight of SWAP70 is 69 kDa. SWAP-70 is a component of an enzyme complex that recombines Ig switch regions in vitro. It is reported that the cloning of the human cDNA and its B lymphocyte-specific expression. Although its sequence contains three nuclear localization signals, in small resting B cells, SWAP-70 is mainly found in the cytoplasm. On stimulation, SWAP-70 translocates to the nucleus. In activated, class-switching B cell cultures, it is associated with membrane IgG, but not IgM. The membrane Ig association requires a functional pleckstrin homology domain and is controlled by the C terminus. It is suggested that SWAP-70 is involved not only in nuclear events but also in signaling in B cell activation (PMID: 10681448). Specification Tested Reactivity: human, mouse Host / Isotype: Rabbit / IgG Class: Recombinant Type: Antibody Immunogen: CatNo: Ag34855 Product name: Recombinant human SWAP70 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 316-550 aa of BC000616 Sequence: HKEARQRRKELRKKQLAEQEELERQMKELQAANESKQQELEAVRKKLEEAASRAAEEEKKRLQTQVELQARFSTELEREKLIRQQMEEQVAQKSSELEQYLQRVRELEDMYLKLQEALEDERQARQDEETVRKLQARLLEEESSKRAELEKWHLEQQQAIQTTEAEKQELENQRVLKEQALQEAMEQLEQLELERKQALEQYEEVKKKLEMATNKTKSWKDKVAHHEGLIRLIEP Predict reactive species Full Name: SWAP-70 protein Calculated Molecular Weight: 69 kDa Observed Molecular Weight: 65-70 kDa GenBank Accession Number: BC000616 Gene Symbol: SWAP70 Gene ID (NCBI): 23075 RRID: AB_3671255 Conjugate: Unconjugated Form: Liquid Purification Method: Protein A purfication UNIPROT ID: Q9UH65 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.