Proteintech, 83648-1-RR, MNT Recombinant monoclonal antibody

Size: 20ul / 100ul The MNT (83648-1-RR) by Proteintech is a Recombinant antibody targeting MNT in WB, IF/ICC, FC (Intra), ELISA applications with reactivity to human, rat samples 83648-1-RR targets MNT in WB, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human, rat samples. Tested Applications Positive WB detected in: HepG2 cells, HeLa cells, HEK-293T cells, Jurkat cells, A431 cells, HT-29 cells, C6 cells Positive IF/ICC detected in: HeLa cells Positive FC (Intra) detected in: A431 cells Recommended dilution Western Blot (WB): WB : 1:5000-1:50000 Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500 Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension Background Information Mnt (Max binding protein), also known as MAD6, ROX, bHLHd3 (class D basic helix-loop-helix protein 3) or MXD6, is a 582 amino acid nuclear protein that forms a complex with Max (Myc-associated factor X) to repress transcription. Mnt contains one basic helix-loop-helix (bHLH) domain and is encoded by a gene that maps to human chromosome 17p13.3. Chromosome 17 comprises over 2.5% of the human genome and encodes over 1,200 genes. Two key tumor suppressor genes are associated with chromosome 17, namely, p53 and BRCA1. Tumor suppressor p53 is necessary for maintenance of cellular genetic integrity by moderating cell fate through DNA repair versus cell death. Like p53, BRCA1 is directly involved in DNA repair, though specifically it is recognized as a genetic determinant of early onset breast cancer and predisposition to cancers of the ovary, colon, prostate gland and fallopian tubes. Specification Tested Reactivity: human, rat Host / Isotype: Rabbit / IgG Class: Recombinant Type: Antibody Immunogen: CatNo: Ag34529 Product name: Recombinant human MNT protein Source: e coli. -derived, PET28a Tag: 6*His Domain: 233-582 aa of BC117563 Sequence: RAHLKECFETLKRNIPNVDDKKTSNLSVLRTALRYIQSLKRKEKEYEHEMERLAREKIATQQRLAELKHELSQWMDVLEIDRVLRQTGQPEDDQASTSTASEGEDNIDEDMEEDRAGLGPPKLSHRPQPELLKSTLPPPSTTPAPLPPHPHPHPHSVALPPAHLPVQQQQPQQKTPLPAPPPPPAAPAQTLVPAPAHLVATAGGGSTVIAHTATTHASVIQTVNHVLQGPGGKHIAHIAPSAPSPAVQLAPATPPIGHITVHPATLNHVAHLGSQLPLYPQPVAVSHIAHTLSHQQVNGTAGLGPPATVMAKPAVGAQVVHHPQLVGQTVLNPVTMVTMPSFPVSTLKLA Predict reactive species Full Name: MAX binding protein Calculated Molecular Weight: 582 aa, 62 kDa Observed Molecular Weight: 70 kDa GenBank Accession Number: BC117563 Gene Symbol: MNT Gene ID (NCBI): 4335 RRID: AB_3671257 Conjugate: Unconjugated Form: Liquid Purification Method: Protein A purification UNIPROT ID: Q99583 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.